Sigrid Haugen's dissertation shows the effect of adiponectin and adiponectin's receptor agonist ADP355 on bone cells and bones, and that local injection of adiponectin can affect orthodontic tooth transfer and was successfully defended on September 11th.
Adiponectin can modify bone growth by increasing the number of human osteoblasts and in addition increasing the expression of markers for early osteoblastogenesis. Adiponectin may also affect bone degradation as it increased the number of osteoclasts but did not affect the activity of the osteoclasts. Adiponectin was associated with a more flexible bone, which was supported by rat bone analyzes and by a 3-dimensional bone model of primary human osteoblasts and osteoclasts in vitro .
In a treatment strategy, a peptide would be advantageous to use over a large complex protein, and therefore the effect of ADP355 was analyzed. ADP355 has been shown to bind the same receptor as adiponectin, AdiopR1, but the effect of ADP355 and adiponectin was not similar in primary human osteoblasts. ADP355 produced less differentiation of the osteoblasts compared to adiponectin. Furthermore, our data showed that adiponectin decreased, while ADP355 increased the expression of AdipoR1 in primary human osteoblasts, which may explain why there was a different response to the cells. In addition, we observed donor variations in the levels of adiponectin receptors ( AdipoR1 and AdipoR2), and how these were affected by treatment with either peptide or protein. In a rat study, topical administration of adiponectin reduced experimental tooth displacement compared to control.
Published papers inculded in Sigrid's thesis
Adiponectin prevents orthodontic tooth movement in rats
The evaluation committee
1st opponent: Professor Martyn Cobourne, Guy's and St Thomas' NHS Foundation Trust, King's College London, UK
2nd opponent: Professor Therese Standal, Center of Molecular Inflammation Research, NTNU, Trondheim
3rd opponent: Professor Anne Merete Aass, Department of Clinical Dentistry, University of Oslo
Main supervisor
Professor Janne Reseland, Department of Clinical Dentistry, University of Oslo
