Håvard Hynne

• Fineide, Fredrik; Tashbayev, Behzod; Elgstøen, Katja B. Prestø; Mørk Sandås, Elise; Rootwelt, Helge & Hynne, Håvard [Show all 12 contributors for this article] (2023). Tear and Saliva Metabolomics in Evaporative Dry Eye Disease in Females. Metabolites. ISSN 2218-1989. 13(11). doi: 10.3390/metabo13111125. Full text in Research Archive
• Frigaard, Julie Mari; Hynne, Håvard & Jensen, Janicke Liaaen (2023). Development and Proposal of a Novel Scoring System to Classify Dry Mouth Severity. Applied Sciences. ISSN 2076-3417. 13(21). doi: 10.3390/app132111758. Full text in Research Archive
• Frigaard, Julie Mari; Hynne, Håvard; Randsborg, Karoline; Mellin-Olsen, Tonje; Hofgaard, Anne Kristine & Young, Alix [Show all 10 contributors for this article] (2023). Exploring oral health indicators, oral health-related quality of life and nutritional aspects in 23 medicated patients from a short-term psychiatric ward. Frontiers in Public Health. ISSN 2296-2565. 11. doi: 10.3389/fpubh.2023.1083256. Full text in Research Archive
• Hynne, Håvard; Tashbayev, Behzod; Diep, My Tien; Sødal, Anne Thea Tveit; Badian, Reza & Chen, Xiangjun [Show all 10 contributors for this article] (2022). The relationship between ocular and oral dryness in a cohort from the 65-year-old population in Norway. Scientific Reports. ISSN 2045-2322. 12(1). doi: 10.1038/s41598-022-13985-6. Full text in Research Archive Show summary

  In the present study, the relationship between dry eyes and dry mouth was explored in 150 65-year-old subjects randomly selected from the general population in Oslo, Norway. The number of drugs, including xerogenic drugs, and current and previous systemic diseases were recorded. Ocular parameters recorded were the McMonnies Dry Eye Questionnaire, the Ocular Surface Disease Index, the Schirmer I Test, tear film break-up time and ocular surface staining. The oral parameters were xerostomia frequency, Summated Xerostomia Inventory, Clinical Oral Dryness Score, and unstimulated and stimulated whole saliva. The participants with current or previous systemic diseases had significantly more ocular and oral symptoms and significantly more oral clinical findings than the participants without a history of disease. Moreover, correlation and factor analyses demonstrated an association between subjective ocular and oral parameters. A significant correlation between the total number of drugs and the presence of ocular and oral symptoms was also noted. When the participants were categorized based on their ocular symptoms, poorer values were found for the oral parameters among the participants more troubled with dry eyes. The results in the present study call for increased awareness and an interdisciplinary approach in matters related to dry eyes and dry mouth.

• Hynne, Håvard; Aqrawi, Lara A.; Jensen, Janicke Liaaen; Thiede, Bernd; Palm, Øyvind & Amdal, Cecilie Delphin [Show all 10 contributors for this article] (2022). Proteomic Profiling of Saliva and Tears in Radiated Head and Neck Cancer Patients as Compared to Primary Sjögren’s Syndrome Patients. International Journal of Molecular Sciences. ISSN 1661-6596. 23(7). doi: 10.3390/ijms23073714. Full text in Research Archive Show summary

  Patients with head and neck cancer (HNC) and patients with primary Sjögren’s syndrome (pSS) may exhibit similar symptoms of dry mouth and dry eyes, as a result of radiotherapy (RT) or a consequence of disease progression. To identify the proteins that may serve as promising disease biomarkers, we analysed saliva and tears from 29 radiated HNC patients and 21 healthy controls, and saliva from 14 pSS patients by mass spectrometry-based proteomics. The study revealed several upregulated, and in some instances overlapping, proteins in the two patient groups. Histone H1.4 and neutrophil collagenase were upregulated in whole saliva of both patient groups, while caspase-14, histone H4, and protein S100-A9 were upregulated in HNC saliva only. In HCN tear fluid, the most highly upregulated protein was mucin-like protein 1. These overexpressed proteins in saliva and tears play central roles in inflammation, host cell injury, activation of reactive oxygen species, and tissue repair. In conclusion, the similarities and differences in overexpressed proteins detected in saliva from HNC and pSS patients may contribute to the overall understanding of the different pathophysiological mechanisms inducing dry mouth. Thus, the recurring proteins identified could possibly serve as future promising biomarkers

• Chen, Xiangjun; Badian, Reza; Hynne, Håvard; Tashbayev, Behzod; Hove, Lene Hystad & Jensen, Janicke Liaaen [Show all 7 contributors for this article] (2022). Morphology of Meibomian Glands in A 65‐Year‐Old Norwegian Population without Dry Eye Disease. Journal of Clinical Medicine. ISSN 2077-0383. 11(3). doi: 10.3390/jcm11030527. Full text in Research Archive Show summary

  Analyses of meibography may help in the diagnosis, prevention, and management of meibomian gland dysfunction (MGD). However, there is currently a paucity of data regarding meibography analyses in the young elderly populations in the Nordic countries. In the current study, meibography of the upper and lower eyelids of 117 65-year-old residents in Oslo, Norway, who did not fulfil the diagnosis of dry eye disease (DED) were analysed. Meibomian gland (MG) dropout and tarsal areas were measured semi-automatically using ImageJ software. The relationship between morphological features of the MGs and clinical dry eye tests was examined. The median percent MG dropout was 26.1% and 40.7% in the upper and lower eyelids, respectively. There was no significant difference between males and females. None of the MG morphological parameters demonstrated significant values in discriminating abnormal dry eye symptom loads or MGD diagnosis from the normal loads. We therefore concluded that moderate MG atrophy was common among the Norwegian population of 65-year-olds without DED and showed no sexual differences. Meibography alone cannot discriminate MGD from non-MGD; thus, both morphological and functional MG tests are necessary when screening for MGD.

• Hynne, Håvard; Mørk Sandås, Elise; Elgstøen, Katja B. Prestø; Rootwelt, Helge; Utheim, Tor Paaske & Galtung, Hilde [Show all 7 contributors for this article] (2022). Saliva Metabolomics in Dry Mouth Patients with Head and Neck Cancer or Sjögren’s Syndrome. Cells. ISSN 2073-4409. 11(3). doi: 10.3390/cells11030323. Full text in Research Archive Show summary

  The etiology of dry mouth conditions is multi-faceted. Patients radiated after head and neck cancer (HNC) and those with primary Sjögren’s syndrome (pSS) share many of the same symptoms despite different causes. With the aim of better understanding the pathophysiology and biochemical processes behind dry mouth with different etiologies, we investigated the metabolic profile of 10 HNC patients, 9 pSS patients and 10 healthy controls using high-performance liquid chromatography-high resolution mass spectrometry (HPLC-MS) metabolomics. Principal component analysis (PCA) revealed different metabolic profiles when comparing all subjects included in the study. Both patient groups showed higher ratios of several pyrimidine nucleotides and nucleosides when compared to controls. This finding may indicate that purinergic signaling plays a role in dry mouth conditions. Moreover, significantly increased levels of DL-3-aminoisobutyric acid were found in HNC patients when compared to controls, and a similar tendency was observed in the pSS patients. Furthermore, a dysregulation in amino acid metabolism was observed in both patient groups. In conclusion, metabolomics analysis showed separate metabolic profiles for HNC and pSS patients as compared to controls that could be useful in diagnostics and for elucidating the different pathophysiologies. The demonstrated dysregulation of pyrimidine nucleotides and levels of metabolites derived from amino acids in the patient groups should be studied further.

• Chen, Xiangjun; Badian, Reza; Hynne, Håvard; Amdal, Cecilie Delphin; Herlofson, Bente Brokstad & Utheim, Øygunn Aass [Show all 10 contributors for this article] (2021). Alterations in meibomian glands in patients treated with intensity-modulated radiotherapy for head and neck cancer. Scientific Reports. ISSN 2045-2322. 11:22419, p. 1–7. doi: 10.1038/s41598-021-01844-9. Full text in Research Archive
• Westgaard, Kristine Løken; Hynne, Håvard; Amdal, Cecilie Delphin; Young, Alix; Singh, Preet Bano & Chen, Xiangjun [Show all 12 contributors for this article] (2021). Oral and ocular late effects in head and neck cancer patients treated with radiotherapy. Scientific Reports. ISSN 2045-2322. 11:4026, p. 1–11. doi: 10.1038/s41598-021-83635-w. Full text in Research Archive
• Aqrawi, Lara A.; Chen, Xiangjun; Hynne, Håvard; Amdal, Cecilie Delphin; Reppe, Sjur & Aass, Hans Christian Dalsbotten [Show all 14 contributors for this article] (2020). Cytokines Explored in Saliva and Tears from Radiated Cancer Patients Correlate with Clinical Manifestations, Influencing Important Immunoregulatory Cellular Pathways. Cells. ISSN 2073-4409. 9(9). doi: 10.3390/cells9092050. Full text in Research Archive

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• Hynne, Håvard; Frigaard, Julie Mari & Jensen, Janicke Liaaen (2024). Dry mouth severity score .
• Frigaard, Julie Mari; Jensen, Janicke Liaaen & Hynne, Håvard (2023). Development of a novel scoring system to classify dry mouth severity.
• Frigaard, Julie Mari; Hynne, Håvard; Randsborg, Karoline; Mellin-Olsen, Tonje; Young, Alix & Rykke, Morten [Show all 10 contributors for this article] (2022). Oral health indicators, oral health-related quality of life and nutritional aspects in medicated patients suffering from anxiety, psychosis, and/or depression. Frontiers in Public Health. ISSN 2296-2565.
• Frigaard, Julie Mari; Hynne, Håvard; Young, Alix; Hove, Lene Hystad; Rykke, Morten & Singh, Preet Bano [Show all 7 contributors for this article] (2022). Do medicated psychiatric patients differ from other dry mouth patients? .
• Hynne, Håvard; Sandås, Elise Mørk; Elgstøen, Katja B. Prestø; Rootwelt, Helge; Galtung, Hilde Kanli & Utheim, Tor Paaske [Show all 7 contributors for this article] (2021). Metabolomics Identify Patterns Indicating Potential Biomarkers in Primary Sjögren’s Syndrome .
• Hynne, Håvard; Aqrawi, Lara A.; Chen, Xiangjun; Amdal, Cecilie Delphin; Reppe, Sjur & Aass, Hans Christian Dalsbotten [Show all 14 contributors for this article] (2021). Cytokine profiling, clinical oral and ocular manifestations post radiotherapy .
• Jensen, Janicke Liaaen; Chen, Xiangjun; Hynne, Håvard; Westgaard, Kristine Løken; Hove, Lene Hystad & Young, Alix [Show all 14 contributors for this article] (2019). Sjögren’s versus irradiated head-neck cancer patients: oral and ocular investigations.
• Hynne, Håvard; Westgaard, Kristine Løken; Herlofson, Bente Brokstad; Amdal, Cecilie Delphin; Hove, Lene Hystad & Young, Alix [Show all 10 contributors for this article] (2019). Oral and Ocular Findings in Radiated Head-and Neck Cancer Patients.
• Young, Alix; Singh, Preet Bano; Hynne, Håvard; Løken Westergaard, Kristine; Hove, Lene Hystad & Rykke, Morten [Show all 10 contributors for this article] (2019). Oral Investigations in Head and Neck Cancer Patients after Radiotherapy. Show summary

  Objectives: To investigate oral complaints and findings in head and neck cancer (HNC) patients who had received radiotherapy and compare them with controls. Methods: As part of a larger study, 30 HNC patients (14 female, age 64.2±9.6y) and 26 age- and sex-matched controls (13 female, 62.9±12.9y, p=0.850) were examined at the Dry Mouth Clinic, Faculty of Dentistry, Oslo. Oral health-related quality of life (OHRQoL) was assessed using the short form Oral Health Impact Profile (OHIP-14: high score-low OHRQoL). Self-reported dysgeusia (yes/no) and sense of taste (10-point VAS: high score-good smell sense) were recorded. Taste scores were recorded using taste strips (high score-good taste function). Oral candida counts were determined from cheek and tongue swabs (0=no growth, 1=minimal, 2=moderate, 3=severe). Volatile sulphur compounds (VSC) in mouth air samples were determined by gas chromatography and related to perceptive threshold-levels (H2S≤112ppb, CH3SH≤26ppb). Between-group comparisons were performed using Mann-Whitney U test and Chi-square test. Results: The HNC patients reported significantly poorer OHRQoL than controls (OHIP sum-score: 18.4±12.4 vs 1.2±2.0, p<0.001). They reported significantly poorer sense of taste compared to controls (6.4±2.4 vs 7.8±1.9, p=0.018), and more often dysgeusia (26.7% vs 7.7%, p=0.087). The measured gustatory scores (20.4±6.4 vs 22.3±4.6, p=0.315) were similar for both groups. Candida scores were significantly higher in the patient group (1.6±1.3 vs 0.7±0.9, p=0.024). The percentage of HNC patients who had oral H2S above the threshold level was significantly lower than for the controls (0% vs 27%, p<0.05). This was not the case for CH3SH (5.5% vs 27%, p=0.105). Conclusion: After radiotherapy, these HNC patients experienced negative oral consequences of the treatment. They reported poorer OHRQoL, and had reduced taste sensation and higher oral candida counts. However, the underlying reason for less problems with halitosis in HNC patients than in controls is worth further exploration.

• Hynne, Håvard; Tashbayev, Behzod; Young, Alix; Herlofson, Bente Brokstad; Hove, Lene Hystad & Rykke, Morten [Show all 14 contributors for this article] (2018). Evaluation of Dry Eye Disease, Oral Dryness and Inflammatory Cytokines in Primary Sjögren´s Syndrome.

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