Håvard Jostein Haugen

• Hussain, Badra; Grytten, Jostein Ivar; Rongen, Gunnar; Sanz Alonso, Mariano & Haugen, Håvard Jostein (2024). Surface Topography has less Influence on Peri-Implantitis than Patient Factors: A Comparative Clinical Study of Two Dental Implant Systems. ACS Biomaterials Science and Engineering. ISSN 2373-9878. doi: 10.1021/acsbiomaterials.3c01809.
• Marovic, Danijela; Bota, Maria; Tarle, Frano; Par, Matej; Haugen, Håvard Jostein & Zheng, Kai [Vis alle 13 forfattere av denne artikkelen] (2024). The influence of copper-doped mesoporous bioactive nanospheres on the temperature rise during polymerization, polymer cross-linking density, monomer release and embryotoxicity of dental composites. Dental Materials. ISSN 0109-5641. doi: 10.1016/j.dental.2024.05.012.
• Alonso, Iván; Haugen, Håvard Jostein; Parreiras Nogueira, Liebert; López-Álvarez, Miriam; González, Pío & López-Peña, Mónica [Vis alle 8 forfattere av denne artikkelen] (2024). Enhanced Bone Healing in Critical-Sized Rabbit Femoral Defects: Impact of Helical and Alternate Scaffold Architectures. Polymers. ISSN 2073-4360. 16(9). doi: 10.3390/polym16091243. Fulltekst i vitenarkiv Vis sammendrag

  This study investigates the effect of scaffold architecture on bone regeneration, focusing on 3D-printed polylactic acid-bioceramic calcium phosphate (PLA-bioCaP) composite scaffolds in rabbit femoral condyle critical defects. We explored two distinct scaffold designs to assess their influence on bone healing and scaffold performance. Structures with alternate (0° /90°) and helical (0°/45°/90°/135°/180°) laydown patterns were manufactured with a 3D printer using a fused deposition modeling technique. The scaffolds were meticulously characterized for pore size, strut thickness, porosity, pore accessibility, and mechanical properties. The in vivo efficacy of these scaffolds was evaluated using a femoral condyle critical defect model in eight skeletally mature New Zealand White rabbits. Then, the results were analyzed micro-tomographically, histologically, and histomorphometrically. Our findings indicate that both scaffold architectures are biocompatible and support bone formation. The helical scaffolds, characterized by larger pore sizes and higher porosity, demonstrated significantly greater bone regeneration than the alternate structures. However, their lower mechanical strength presented limitations for use in load-bearing sites.

• Øvrebø, Øystein; Giorgi, Zoe; De Lauretis, Angela; Vanoli, Valeria; Castiglione, Franca & Briatico-Vangosa, Francesco [Vis alle 10 forfattere av denne artikkelen] (2024). Characterisation and biocompatibility of crosslinked hyaluronic acid with BDDE and PEGDE for clinical applications. Reactive & functional polymers. ISSN 1381-5148. 200. doi: 10.1016/j.reactfunctpolym.2024.105920. Fulltekst i vitenarkiv
• Øvrebø, Øystein; De Lauretis, Angela; Ma, Qianli; Perale, Giuseppe; Nilsen, Ola & Rossi, Filippo [Vis alle 7 forfattere av denne artikkelen] (2024). Towards bone regeneration: Understanding the nucleating ability of proline-rich peptides in biomineralisation. Biomaterials Advances. ISSN 2772-9516. 159. doi: 10.1016/j.bioadv.2024.213801. Fulltekst i vitenarkiv
• Haugen, Håvard Jostein & de Lange, Thomas (2024). Multiple choice as formative assessment in dental education. European journal of dental education. ISSN 1396-5883. 00, s. 1–13. doi: 10.1111/eje.13002. Fulltekst i vitenarkiv Vis sammendrag

  Introduction: The effectiveness of Multiple-Choice Questions (MCQs) in dental education is pivotal to student performance and knowledge advancement. However, their optimal implementation requires exploration to enhance the benefits. Materials and Methods: An educational tool incorporating MCQs was administered from the 5th to the 10th semester in a dental curriculum. The students filled out a questionnaire after the MCQ, which was linked to the Learning Management System. 4 cohorts of 4 semesters generated 2300 data points analysed by Spearmen correlation and mixed model regression analysis. Results demonstrated a significant correlation between early exam preparation and improved student performance. Independent study hours and lecture attendance emerged as significant predictors, accounting for approximately 10.27% of the variance in student performance on MCQs. While the number of MCQs taken showed an inverse relationship with study hours, the perceived clarity of these questions positively correlated with academic achievement. Conclusion: MCQs have proven effective in enhancing student learning and knowledge within the discipline. Our analysis underscores the important role of independent study and consistent lecture attendance in positively influencing MCQ scores. The study provides valuable insights into using MCQs as a practical tool for dental student learning. Moreover, the clarity of assessment tools, such as MCQs, remains pivotal in influencing student outcomes. This study underscores the multifaceted nature of learning experiences in dental education and the importance of bridging the gap between student expectations and actual performance.

• De Lauretis, Angela; Øvrebø, Øystein; Romandini, Mario; Lyngstadaas, Ståle Petter; Rossi, Filippo & Haugen, Håvard Jostein (2024). From Basic Science to Clinical Practice: A Review of Current Periodontal/Mucogingival Regenerative Biomaterials. Advanced Science. ISSN 2198-3844. doi: 10.1002/advs.202308848. Fulltekst i vitenarkiv
• Hussain, Badra; Simm, Roger; Bueno, Jaime; Giannettou, Savvas; Naemi, Ali-Oddin & Lyngstadaas, Ståle Petter [Vis alle 7 forfattere av denne artikkelen] (2024). Biofouling on titanium implants: a novel formulation of poloxamer and peroxide for in situ removal of pellicle and multi-species oral biofilm. Regenerative Biomaterials. ISSN 2056-3426. 11. doi: 10.1093/rb/rbae014. Fulltekst i vitenarkiv
• Boix Lemonche, Gerard; Hildebrand, Torben; Haugen, Håvard Jostein; Petrovski, Goran & Parreiras Nogueira, Liebert (2024). Contrast-enhanced Micro-CT 3D visualization of cell distribution in hydrated human cornea. Heliyon. ISSN 2405-8440. 10(3). doi: 10.1016/j.heliyon.2024.e25828. Fulltekst i vitenarkiv
• Korkeamäki, Jannika Timontytär; Rashad, Ahmad; Berstad, Kaia; Weber, Florian; Syverud, Kristin & Haugen, Håvard Jostein [Vis alle 7 forfattere av denne artikkelen] (2024). Biomimetic highly porous nanocellulose–nanohydroxyapatite scaffolds for bone tissue engineering. Cellulose. ISSN 0969-0239. doi: 10.1007/s10570-024-05732-z. Fulltekst i vitenarkiv
• Schot, Maik; Becker, Malin; Paggi, Carlo Alberto; Gomes, Francisca; Koch, Timo & Gensheimer, Tarek [Vis alle 12 forfattere av denne artikkelen] (2023). Photoannealing of Microtissues Creates High-Density Capillary Network Containing Living Matter in a Volumetric-Independent Manner. Advanced Materials. ISSN 0935-9648. doi: 10.1002/adma.202308949. Fulltekst i vitenarkiv Vis sammendrag

  The vascular tree is crucial for the survival and function of large living tissues. Despite breakthroughs in 3D bioprinting to endow engineered tissues with large blood vessels, there is currently no approach to engineer high-density capillary networks into living tissues in a scalable manner. We here present photo-annealing of living microtissues(PALM) as a scalable strategy to engineer capillary-rich tissues. Specifically, in-air microfluidics was used to produce living microtissues composed of cell-laden microgels in ultra-high throughput, which could be photo-annealed into a monolithic living matter. Annealed microtissues inherently give rise to an open and interconnected pore network within the resulting living matter. Interestingly, utilizing soft microgels enables microgel deformation, which leads to the uniform formation of capillary-sized pores. Importantly, the ultra-high throughput nature underlying the microtissue formation uniquely facilitates scalable production of living tissues of clinically relevant sizes (>1 cm3 ) with an integrated high-density capillary network. In short, PALM generates monolithic, microporous, modular tissues that meet the previously unsolved need for large engineered tissues containing high-density vascular networks, which is anticipated to advance the fields of engineered organs, regenerative medicine, and drug screening

• Goa, Hui; Jiang, Nan; Niu, Qiannan; Mei, Shenglin; Haugen, Håvard Jostein & Ma, Qianli (2023). Biocompatible nanostructured silver-incorporated implant surfaces show effective antibacterial, osteogenic, and anti-inflammatory effects in vitro and in rat model. International Journal of Nanomedicine. ISSN 1176-9114. 18, s. 7359–7378. doi: 10.2147/IJN.S435415. Fulltekst i vitenarkiv
• Sevgi, Usame; Johnsen, Gaute Floer; Hussain, Badra; Piasecki, Lucila; Parreiras Nogueira, Liebert & Haugen, Håvard Jostein (2023). Morphometric micro-CT study of contralateral mandibular incisors. Clinical Oral Investigations. ISSN 1432-6981. 28(1). doi: 10.1007/s00784-023-05419-y. Fulltekst i vitenarkiv Vis sammendrag

  Objectives: This study aimed to determine the degree of similarity and symmetry in the anatomy of contralateral mandibular incisors. Three-dimensional (3D) models of extracted teeth were obtained from microtomography (micro-CT) scans. Qualitative and quantitative assessments of the morphology and comparison of contralateral pairs were made. The null hypothesis was that contralateral mandibular incisors could not be considered identical in simple morphometric measurements. Methods: Sixty pairs of mandibular incisors were extracted from 30 patients and scanned with micro-CT. Virtual models of the cemento-enamel junction to the root apex were rendered. Parameters such as length, canal width, dentinal thicknesses, tortuosity, centerline length, accessory canals, root canal configurations, and root canal orifice cross-sections were used to compare the teeth. Width and thickness comparisons between paired teeth in the same individual were made by paired t-test (Wilcoxon Signed Rank Test for variables not normally distributed). An online randomization tool generated randomized pairs (independent of the individual/patient). Subsequently, an unpaired t-test (or Mann-Whitney U test for non-normally distributed parameters) and a correlation analysis were conducted. Canal configurations were classified according to preexisting classification schemes. The number and location of accessory canals and apical foramina were registered and compared. Results: Utilizing advanced imaging techniques and quantitative analyses, our study establishes that contralateral mandibular incisors exhibit a remarkable degree of symmetry in multiple morphological parameters, including length, canal width, and dentinal thicknesses The apical third showed a high degree of inter-variability for the contralateral pairs. The rigorous statistical analysis of the normalized parameters by Z score showed no statistically significant differences between the contralateral mandibular incisors. Comparisons between central and lateral teeth revealed differences in root length but no significant disparity in the distribution of accessory canals. Central teeth, on average, were longer, while accessory canals were distributed relatively evenly between central and lateral teeth. Conclusions: The findings of this study further establish the significant similarities between contralateral mandibular incisors, reinforcing their suitability as a reliable substrate for root canal comparison studies. Clinical Relevance: The absence of statistically significant differences between contralateral pairs in normalized parameters underscores their potential as a reliable reference point for root canal comparison studies in clinical dentistry. Furthermore, our findings emphasize the importance of individualized treatment planning, considering the natural symmetry in mandibular incisors to enhance clinical decision-making. This research contributes valuable insights to the field of endodontics, offering a standardized approach to sample selection and enriching the understanding of dental anatomy

• Mateu-Sanz, Miguel; Fuenteslópez, Carla V.; Uribe-Gomez, Juan; Haugen, Håvard Jostein; Pandit, Abhay & Ginebra, Maria-Pau [Vis alle 9 forfattere av denne artikkelen] (2023). Redefining biomaterial biocompatibility: challenges for artificial intelligence and text mining. Trends in Biotechnology. ISSN 0167-7799. doi: 10.1016/j.tibtech.2023.09.015. Fulltekst i vitenarkiv
• Abadpour, Shadab; Niemi, Essi Maria; Strid Orrhult, Linnea; Hermanns, Carolin; de Vries, Rick & Parreiras Nogueira, Liebert [Vis alle 12 forfattere av denne artikkelen] (2023). Adipose-Derived Stromal Cells Preserve Pancreatic Islet Function in a Transplantable 3D Bioprinted Scaffold. Advanced Healthcare Materials. ISSN 2192-2640. 12(32). doi: 10.1002/adhm.202300640. Fulltekst i vitenarkiv
• Miri, Zahra; Haugen, Håvard Jostein; Loča, Dagnija; Rossi, Filippo; Perale, Giuseppe & Moghanian, Amirhossein [Vis alle 7 forfattere av denne artikkelen] (2023). Review on the strategies to improve the mechanical strength of highly porous bone bioceramic scaffolds. Journal of the European Ceramic Society. ISSN 0955-2219. doi: 10.1016/j.jeurceramsoc.2023.09.003. Fulltekst i vitenarkiv
• Hildebrand, Torben; Novak, Jan; Parreiras Nogueira, Liebert; Boccaccini, Aldo Roberto & Haugen, Håvard Jostein (2023). Durability assessment of hydrogel mountings for contrast-enhanced micro-CT. Micron. ISSN 0968-4328. 174. doi: 10.1016/j.micron.2023.103533. Fulltekst i vitenarkiv Vis sammendrag

  Micro-computed tomography (micro-CT) provides valuable data for studying soft tissue, though it is often affected by sample movement during scans and low contrast in X-ray absorption. This can result in lower image quality and geometric inaccuracies, collectively known as 'artefacts'. To mitigate these issues, samples can be embedded in hydrogels and enriched with heavy metals for contrast enhancement. However, the long-term durability of these enhancements remains largely unexplored. In this study, we examine the effects of two contrast enhancement agents – iodine and phosphotungstic acid (PTA) – and two hydrogels – agarose and Poloxamer 407 – over a 14-day period. We used Drosophila melanogaster as a test model for our investigation. Our findings reveal that PTA and agarose are highly durable, while iodine and poloxamer hydrogel exhibits higher leakage rates. These observations lay the foundation for estimating contrast stabilities in contrast-enhanced micro-CT with hydrogel embedding and serve to inform future research in this field.

• Ning, Ke; Fang, Yang; Bronkhorst, Ewald M.; Ruben, Jan; Parreiras Nogueira, Liebert & Haugen, Håvard Jostein [Vis alle 8 forfattere av denne artikkelen] (2023). Fatigue behaviour of a self-healing dental composite. Dental Materials. ISSN 0109-5641. 39(10), s. 913–921. doi: 10.1016/j.dental.2023.08.172. Fulltekst i vitenarkiv
• Munir, Arooj; Reseland, Janne Elin; Tiainen, Hanna; Haugen, Håvard Jostein; Sikorski, Pawel & Christiansen, Emil [Vis alle 9 forfattere av denne artikkelen] (2023). Osteocyte-Like Cells Differentiated From Primary Osteoblasts in an Artificial Human Bone Tissue Model. JBMR Plus. ISSN 2473-4039. s. 1–10. doi: 10.1002/jbm4.10792. Fulltekst i vitenarkiv
• Pinelli, Filippo; Ponti, Maddalena; Delleani, Sara; Pizzetti, Fabio; Vanoli, Valeria & Vangosa, Francesco Briatico [Vis alle 12 forfattere av denne artikkelen] (2023). β-Cyclodextrin functionalized agarose-based hydrogels for multiple controlled drug delivery of ibuprofen. International Journal of Biological Macromolecules. ISSN 0141-8130. 252. doi: 10.1016/j.ijbiomac.2023.126284.
• Miri, Zahra; Farè, Silvia; Ma, Qianli & Haugen, Håvard Jostein (2023). Updates on polyurethane and its multifunctional applications in biomedical engineering. Progress in Biomedical Engineering. 5(4). doi: 10.1088/2516-1091/acef84. Fulltekst i vitenarkiv
• Hussain, Badra; Khan, Sadia Nazir; Agger, Anne Eriksson; Ellingsen, Jan Eirik; Lyngstadaas, Ståle Petter & Bueno, Jaime [Vis alle 7 forfattere av denne artikkelen] (2023). A Comparative Investigation of Chemical Decontamination Methods for In-Situ Cleaning of Dental Implant Surfaces. Journal of Functional Biomaterials. ISSN 2079-4983. 14(8). doi: 10.3390/jfb14080394. Fulltekst i vitenarkiv
• Alonso, Iván; Haugen, Håvard Jostein; López-Peña, Mónica; Cantalapiedra, Antonio Gonzalez & Muñoz, Fernando (2023). Use of 3D-printed polylactic acid/bioceramic composite scaffolds for bone tissue engineering in preclinical in vivo studies: A systematic review. Acta Biomaterialia. ISSN 1742-7061. 168, s. 1–21. doi: 10.1016/j.actbio.2023.07.013. Fulltekst i vitenarkiv
• Moore, Axel C; Hennessy, Matthew G; Parreiras Nogueira, Liebert; Franks, Susan J; Taffetani, Matteo & Seong, Hyejeong [Vis alle 17 forfattere av denne artikkelen] (2023). Fiber Reinforced Hydrated Networks Recapitulate the Poroelastic Mechanics of Articular Cartilage . Acta Biomaterialia. ISSN 1742-7061. doi: 10.1016/j.actbio.2023.06.015. Fulltekst i vitenarkiv Vis sammendrag

  The role of poroelasticity on the functional performance of articular cartilage has been established in the scientific literature since the 1960s. Despite the extensive knowledge on this topic there remain few attempts to design for poroelasticity and to our knowledge no demonstration of an engineered poroelastic material that approaches the physiological performance. In this paper, we report on the development of an engineered material that begins to approach physiological poroelasticity. We quantify poroelasticity using the fluid load fraction, apply mixture theory to model the material system, and determine cytocompatibility using primary human mesenchymal stem cells. The design approach is based on a fiber reinforced hydrated network and uses routine fabrication methods (electrohydrodynamic deposition) and materials (poly[ɛ-caprolactone] and gelatin) to develop the engineered poroelastic material. This composite material achieved a mean peak fluid load fraction of 68%, displayed consistency with mixture theory, and demonstrated cytocompatibility. This work creates a foundation for designing poroelastic cartilage implants and developing scaffold systems to study chondrocyte mechanobiology and tissue engineering.

• Sæter, Torstein; Galland, Olivier; Feneuil, Blandine; Haugen, Håvard Jostein & Carlson, Andreas (2023). Growth of a viscoplastic blister underneath an elastic sheet. Journal of Fluid Mechanics. ISSN 0022-1120. 964, s. 1–16. doi: 10.1017/jfm.2023.320. Fulltekst i vitenarkiv
• Chen, Song; Liu, Dachuan; Le, Fu; Bing, Ni; Zongkun, Chen & Knaus, Jennifer [Vis alle 12 forfattere av denne artikkelen] (2023). Formation of amorphous iron-calcium phosphate with high stability. Advanced Materials. ISSN 0935-9648. doi: 10.1002/adma.202301422. Fulltekst i vitenarkiv
• Palombo, David; Rahmati, Maryam; Vignoletti, Fabio; Sanz-Esporrin, Javier; Salido, Mari Paz & Haugen, Håvard Jostein [Vis alle 7 forfattere av denne artikkelen] (2023). Hard and soft tissue healing around teeth prepared with the biologically oriented preparation technique and restored with provisional crowns: An in vivo experimental investigation. Journal of Clinical Periodontology. ISSN 0303-6979. doi: 10.1111/jcpe.13825. Fulltekst i vitenarkiv
• Ma, Qianli; Miri, Zahra; Haugen, Håvard Jostein; Moghanian, Amirhossein & Loca, Dagnija (2023). Significance of mechanical loading in bone fracture healing, bone regeneration, and vascularization. Journal of Tissue Engineering. ISSN 2041-7314. 14. doi: 10.1177/20417314231172573. Fulltekst i vitenarkiv Vis sammendrag

  In 1892, J.L. Wolff proposed that bone could respond to mechanical and biophysical stimuli as a dynamic organ. This theory presents a unique opportunity for investigations on bone and its potential to aid in tissue repair. Routine activities such as exercise or machinery application can exert mechanical loads on bone. Previous research has demonstrated that mechanical loading can affect the differentiation and development of mesenchymal tissue. However, the extent to which mechanical stimulation can help repair or generate bone tissue and the related mechanisms remain unclear. Four key cell types in bone tissue, including osteoblasts, osteoclasts, bone lining cells, and osteocytes, play critical roles in responding to mechanical stimuli, while other cell lineages such as myocytes, platelets, fibroblasts, endothelial cells, and chondrocytes also exhibit mechanosensitivity. Mechanical loading can regulate the biological functions of bone tissue through the mechanosensor of bone cells intraosseously, making it a potential target for fracture healing and bone regeneration. This review aims to clarify these issues and explain bone remodeling, structure dynamics, and mechano-transduction processes in response to mechanical loading. Loading of different magnitudes, frequencies, and types, such as dynamic versus static loads, are analyzed to determine the effects of mechanical stimulation on bone tissue structure and cellular function. Finally, the importance of vascularization in nutrient supply for bone healing and regeneration was further discussed

• Ma, Qianli; Rubenis, Kristaps; Sigurjonsson, Olafur E.; Hildebrand, Torben; Standal, Therese & Zemjane, Signe [Vis alle 9 forfattere av denne artikkelen] (2023). Eggshell-derived amorphous calcium phosphate: Synthesis, characterization and bio-functions as bone graft materials in novel 3D osteoblastic spheroids model. Smart Materials in Medicine. ISSN 2590-1834. 4, s. 522–537. doi: 10.1016/j.smaim.2023.04.001. Fulltekst i vitenarkiv
• Chen, Song; Wang, Huan; Liu, Dachuan; Bai, Jianzhong; Haugen, Håvard Jostein & LI, Bing [Vis alle 7 forfattere av denne artikkelen] (2023). Early osteoimmunomodulation by mucin hydrogels augments the healing and revascularization of rat critical-size calvarial bone defects. Bioactive Materials. ISSN 2452-199X. 25, s. 176–188. doi: 10.1016/j.bioactmat.2023.01.022. Fulltekst i vitenarkiv
• Fu, Zhaoyue; Hou, Yongli; Haugen, Håvard Jostein; Chen, Xutao; Tang, Kang & Fang, Liang [Vis alle 10 forfattere av denne artikkelen] (2023). TiO2 nanostructured implant surface-mediated M2c polarization of inflammatory monocyte requiring intact cytoskeleton rearrangement. Journal of Nanobiotechnology. ISSN 1477-3155. 21. doi: 10.1186/s12951-022-01751-9. Fulltekst i vitenarkiv Vis sammendrag

  Background Microgravity directly disturbs the reorganization of the cytoskeleton, exerting profound effects on the physiological process of macrophages. Although it has been established that macrophage M1/M2 polarization could be manipulated by the surface nanostructure of biomaterial in our previous study under normal gravity, how will inflammatory monocytes (iMos)-derived macrophages respond to diverse nanostructured Ti surfaces under normal gravity or microgravity remains unrevealed. Results In this study, Cytochalasin D, a cytoskeleton relaxant, was employed to establish the simulated microgravity (SMG) environment. Our results showed that human iMos polarized into M2c macrophages on NT5 surface but M1 type on NT20 surface with divergent inflammatory phenotypes according to the profile of macrophage polarization featured molecules under normal gravity. However, such manipulative effects of NTs surfaces on iMos-derived macrophages were strikingly weakened by SMG, characterized by the altered macrophage morphology, changed cytokine secretion profile, and decreased cell polarization capacity. Conclusions To our knowledge, this is the first metallic implantable material study focusing on the functions of specific monocyte subsets and its crucial role of the cytoskeleton in materials-mediated host immune response, which enriches our mechanism knowledge about the crosstalk between immunocytes and biomaterials. The results obtained in the present study may also provide potential targets and strategies for biomaterial development and clinical treatment via precise immune-regulation under normal gravity and microgravity.

• Pietryga, Krzysztof; Reczyńska-Kolman, Katarzyna; Reseland, Janne Elin; Haugen, Håvard Jostein; Larreta-Garde, Véronique & Pamula, Elzbieta (2023). Biphasic monolithic osteochondral scaffolds obtained by diffusion-limited enzymatic mineralization of gellan gum hydrogel. Biocybernetics and Biomedical Engineering (BBE). ISSN 0208-5216. doi: 10.1016/j.bbe.2022.12.009. Fulltekst i vitenarkiv Vis sammendrag

  Biphasic monolithic materials for the treatment of osteochondral defects were produced from polysaccharide hydrogel, gellan gum (GG). GG was enzymatically mineralized by alkaline phosphatase (ALP) in the presence of calcium glycerophosphate (CaGP). The desired distribution of the calcium phosphate (CaP) mineral phase was achieved by limiting the availability of CaGP to specific parts of the GG sample. Therefore, mineralization of GG was facilitated by the diffusion of CaGP, causing the formation of the CaP gradient. The distribution of CaP was analyzed along the cross section of the GG. The formation of a CaP gradient was mainly affected by the mineralization time and the ALP concentration. The formation of CaP was confirmed by Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy and mapping, as well as energy-dispersive X-ray spectroscopy (EDX) mapping of the interphase. The microstructure of mineralized and non-mineralized parts of the material was characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM) showing sub-micrometer CaP crystal formation, resulting in increased surface roughness. Compression tests and rheometric analyzes showed a 10-fold increase in stiffness of GG mineralized part. Concomitantly, micromechanical tests performed by AFM showed an increase of Young's modulus from 17.8 to more than 200 kPa. In vitro evaluation of biphasic scaffolds was performed in contact with osteoblast-like MG-63 cells. The mineralized parts of GG were preferentially colonized by the cells over the non-mineralized parts. The results showed that osteochondral scaffolds of the desired structure and properties can be made from GG using a diffusion-limited enzymatic mineralization method.

• Haugen, Håvard Jostein; Schneider, Armin; Schlicht, Henning; Wu, Hongbin; Doundoulakis, Emmanouil & Wilhelm, Dirk [Vis alle 9 forfattere av denne artikkelen] (2022). Long-term in vivo response of a polyurethane gastric implant for treating gastro-oesophageal reflux diseases-a comparison of different surface treatments. Biomedical Materials & Devices. ISSN 2731-4812. doi: 10.1007/s44174-022-00055-8. Fulltekst i vitenarkiv Vis sammendrag

  Gastro oesophagael reflux disease (GORD) is common in the Western hemisphere. Patients with regurgitated reflux are typically treated with fundoplication surgery. We present a newly designed polyurethane implant which passively aids the sphincter in reducing gastric fluids within the oesophagus. The gastric implant has an open porous inner side which allows for tissue ingrowth from the oesophagus and thus allows for fixation around the sphincter. In addition, a device for minimally invasive surgery of this implant was developed and used in a pig model. The unmodified GORD implant was placed around the pig’s oesophagus with unsatisfactory results, leading to insufficient fixation at the implantation site and scarring tissue leading to dysphagia. In addition, two surface modifications, plasma activation and TiO2 deposition were used to improve the implant’s host tissue response. The biocompatibility effects of the surface treatments and sterilisation method on the implant were investigated in vitro and in vivo. In vitro tests found that the plasma activation and TiO2 deposition have effectively enhanced the surface hydrophilicity and, consequently, the cell response to the implant. In addition, the gamma sterilisation harmed the plasma-activated implant. The plasma activation was more effective than TiO 2 deposition as a surface treatment method for improving the tissue response of this implant in vivo. In addition, the in vivo experiment proved tissue ingrowth as deep as 1 mm into the porous structure of the implant. The GORD implants were encapsulated wholly in fibrous tissue; however, the capsule thickness diminished over time. Finally, the TiO2-coated implants showed the poorest histocompatibility, contradictory to the in vitro findings. This study shows that it is possible to produce a plasma-treated porous polyurethane gastric implant that allows for fibrous tissue ingrowth, reduced in vivo encapsulation, and enhanced chemical properties

• Vanoli, Valeria; Delleani, Sara; Casalegno, Mosè; Pizzetti, Fabio; Makvandi, Pooyan & Haugen, Håvard Jostein [Vis alle 9 forfattere av denne artikkelen] (2022). Hyaluronic acid-based hydrogels: drug diffusion investigated by HR-MAS NMR and release kinetics. Carbohydrate Polymers. ISSN 0144-8617. 301. doi: 10.1016/j.carbpol.2022.120309. Fulltekst i vitenarkiv Vis sammendrag

  Hydrogels based on hyaluronic acid (HA) and agarose–carbomer (AC) units have been prepared and explored as drug delivery systems. The complex architecture of the polymer network, such as mesh size, HA molecular weight and drug-polymer non-covalent interactions across the 3D polymer matrix, strongly influence the release capability/profile of these materials. In this study, High-Resolution Magic Angle Spinning (HR-MAS) NMR Spectroscopy has been used to investigate the transport behaviour of two different drugs, such as ethosuximide (neutral molecule) and sodium salicylate (net negative charge) within the AC and AC-HA hydrogel networks prepared with different mesh sizes. Analysis of the experimental data provides evidence of superdiffusive motion for all formulations containing sodium salicylate, while ethosuximide molecules undergo unrestricted diffusion within the gel matrix. We further speculate that the superdiffusive motion observed at the nanoscale can be responsible for the faster release of sodium salicylate from all hydrogel formulations.

• Munir, Arooj; Marovic, Danijela; Parreiras Nogueira, Liebert; Simm, Roger; Naemi, Ali-Oddin & Landrø, Sander Marius [Vis alle 14 forfattere av denne artikkelen] (2022). Using Copper-Doped Mesoporous Bioactive Glass Nanospheres to Impart Anti-Bacterial Properties to Dental Composites. Pharmaceutics. ISSN 1999-4923. 14(10). doi: 10.3390/pharmaceutics14102241. Fulltekst i vitenarkiv
• Bjørge, Isabel M.; De Sousa, Bárbara M.; Patrício, Sónia G.; Silva, Ana Sofia; Parreiras Nogueira, Liebert & Santos, Lúcia F. [Vis alle 10 forfattere av denne artikkelen] (2022). Bioengineered Hierarchical Bonelike Compartmentalized Microconstructs Using Nanogrooved Microdiscs. ACS Applied Materials & Interfaces. ISSN 1944-8244. 14(17), s. 19116–19128. doi: 10.1021/acsami.2c01161.
• Hongji, Yan; De Deus, Gustavo; Kristoffersen, Ida Marie; Wiig, Elisabeth; Reseland, Janne Elin & Johnsen, Gaute Floer [Vis alle 8 forfattere av denne artikkelen] (2022). Regenerative Endodontics by Cell Homing: A Review of Recent Clinical trials. Journal of Endodontics. ISSN 0099-2399. 49(1), s. 4–17. doi: 10.1016/j.joen.2022.09.008. Fulltekst i vitenarkiv
• Thieu, Minh Khai Le; Stoetzel, Sabine; Rahmati, Maryam; El Khassawna, Thaqif; Verket, Anders & Sanz-Esporrin, Javier [Vis alle 9 forfattere av denne artikkelen] (2022). Immunohistochemical comparison of lateral bone augmentation using a synthetic TiO<inf>2</inf> block or a xenogeneic graft in chronic alveolar defects. Clinical Implant Dentistry and Related Research. ISSN 1523-0899. doi: 10.1111/cid.13143. Fulltekst i vitenarkiv
• Mortier, Claudio; Costa, Dora C.S.; Oliveira, Mariana B; Haugen, Håvard Jostein; Lyngstadaas, Ståle Petter & Blaker, Jonny j [Vis alle 7 forfattere av denne artikkelen] (2022). Advanced hydrogels based on natural macromolecules: chemical routes to achieve mechanical versatility. Materials Today Chemistry. ISSN 2468-5194. 26. doi: 10.1016/j.mtchem.2022.101222. Fulltekst i vitenarkiv
• Partenope, Alessandra; Pizzetti, Fabio; Vanoli, Valeria; Casalegno, Mosè; Cingolani, Alberto & Parreiras Nogueira, Liebert [Vis alle 9 forfattere av denne artikkelen] (2022). A facile surfactant-free strategy to construct porous structures with hydrophobic and hydrophilic domains from polymer/water mixtures. Materials Today Communications. ISSN 2352-4928. 33. doi: 10.1016/j.mtcomm.2022.104290.
• Marovic, Danijela; Par, Matej; Tauböck, Tobias T.; Haugen, Håvard Jostein; Mandic, Visnja Negovetic & Wuethrich, Damian [Vis alle 11 forfattere av denne artikkelen] (2022). Impact of Copper-Doped Mesoporous Bioactive Glass Nanospheres on the Polymerisation Kinetics and Shrinkage Stress of Dental Resin Composites. International Journal of Molecular Sciences. ISSN 1661-6596. 23(15). doi: 10.3390/ijms23158195. Fulltekst i vitenarkiv
• Haugen, Håvard Jostein; Makhtari, Soukayna; Ahmadi, Sara & Hussain, Badra (2022). The Antibacterial and Cytotoxic Effects of Silver Nanoparticles Coated Titanium Implants: A Narrative Review. Materials. ISSN 1996-1944. 15(14), s. 1–19. doi: 10.3390/ma15145025. Fulltekst i vitenarkiv Vis sammendrag

  Nanotechnology has become an emerging research field with numerous biomedical scientific applications. Silver possesses bactericidal activities that have been harnessed for centuries; however, there is a concern about the toxic effects of silver nanoparticles. This paper aims to provide an overview of silver-treated dental implants and discuss their potential to reduce the prevalence of peri-implant diseases. An electronic search was performed using PubMed. After screening, data extraction was performed on the 45 remaining articles using inclusion and exclusion criteria. Most of the articles demonstrated that silver nanoparticles embedded in a coating layer and/or on surfacetreated titanium exhibit sound antibacterial effects and biocompatibility. Most of the reviewed studies revealed that silver nanoparticles on dental implant surfaces reduced cytotoxicity but provided a prolonged antibacterial effect. The cytotoxicity and antibacterial effect are closely linked to how the silver nanoparticles are released from the titanium surfaces, where a slower release increases cell viability and proliferation. However, to improve the clinical translation, there is still a need for more studies, especially evaluating the long-term systemic effects and studies recreating the conditions in the oral cavity.

• Schröder, Maria Elisabeth; Reseland, Janne Elin & Haugen, Håvard Jostein (2022). Osteoblasts in a Perfusion Flow Bioreactor—Tissue Engineered Constructs of TiO2 Scaffolds and Cells for Improved Clinical Performance. Cells. ISSN 2073-4409. 11(13). doi: 10.3390/cells11131995. Fulltekst i vitenarkiv
• Hassan, Hafiz Wajahat; Rahmati, Maryam; Barrantes Bautista, Alejandro; Haugen, Håvard Jostein & Mirtaheri, Peyman (2022). In Vitro Monitoring of Magnesium-Based Implants Degradation by Surface Analysis and Optical Spectroscopy. International Journal of Molecular Sciences. ISSN 1661-6596. 23(11), s. 1–15. doi: 10.3390/ijms23116099. Fulltekst i vitenarkiv Vis sammendrag

  Magnesium (Mg)-based degradable alloys have attracted substantial attention for tissue engineering applications due to their biodegradability and potential for avoiding secondary removal surgeries. However, insufficient data in the existing literature regarding Mg’s corrosion and gas formation after implantation have delayed its wide clinical application. Since the surface properties of degradable materials constantly change after contact with body fluid, monitoring the behavior of Mg in phantoms or buffer solutions could provide some information about its physicochemical surface changes over time. Through surface analysis and spectroscopic analysis, we aimed to investigate the structural and functional properties of degradable disks. Since bubble formation may lead to inflammation and change pH, monitoring components related to acidosis near the cells is essential. To study the bubble formation in cell culture media, we used a newly developed Mg alloy (based on Mg, Zinc and Calcium), pure Mg and commercially available grade 2 Titanium (Ti) disks in Dulbecco's Modified Eagle Medium (DMEM) solution to observe their behavior over ten days of immersion. Using surface analysis, and the information from near-infrared spectroscopy (NIRS), we concluded conditions associated with the medical risks of Mg alloy disintegration. NIRS is used to investigate the degradation behaviour of Mg-based disks in the cell culture media, which is correlated with the surface analysis where possible.

• D. Tien, Nguyen; Geng, Tianxiang; Heyward, Catherine Anne; Reseland, Janne Elin; Lyngstadaas, Ståle Petter & Blaker, Jonathan James [Vis alle 7 forfattere av denne artikkelen] (2022). Solution blow spinning of highly deacetylated chitosan nanofiber scaffolds for dermal wound healing. Materials Science and Engineering C: Materials for Biological Applications. ISSN 0928-4931. 137. doi: 10.1016/j.bioadv.2022.212871. Fulltekst i vitenarkiv
• Kallas, Pawel; Valen, Håkon; Hulander, Mats; Gadegaard, Nikolaj; Stormonth-Darling, John & O'Reilly, Padraic [Vis alle 9 forfattere av denne artikkelen] (2022). Protein-coated nanostructured surfaces affect the adhesion of Escherichia coli. Nanoscale. ISSN 2040-3364. 14(20), s. 7736–7746. doi: 10.1039/d2nr00976e. Fulltekst i vitenarkiv
• Haugen, Håvard Jostein & Chen, Hongyu (2022). Is There a Better Biomaterial for Dental Implants than Titanium?—A Review and Meta-Study Analysis. Journal of Functional Biomaterials. ISSN 2079-4983. 13(2). doi: 10.3390/jfb13020046. Fulltekst i vitenarkiv
• Luzon, Javier; Thorsen, Yngve; Parreiras Nogueira, Liebert; Andersen, Solveig Norheim; Edwin, Bjørn von Gohren & Haugen, Håvard Jostein [Vis alle 8 forfattere av denne artikkelen] (2022). Reconstructing topography and extent of injury to the superior mesenteric artery plexus in right colectomy with extended D3 mesenterectomy: a composite multimodal 3-dimensional analysis. Surgical Endoscopy. ISSN 0930-2794. 36(10), s. 7607–7618. doi: 10.1007/s00464-022-09200-2. Fulltekst i vitenarkiv Vis sammendrag

  Background Superior mesenteric artery plexus (SMAP) injury is reported to cause postoperative intractable diarrhea after pancreatic/colonic surgery with extended lymphadenectomy. This study aims to describe the SMAP microanatomy and extent of injury after right colectomy with extended D3 mesenterectomy for cancer. Methods Three groups (I) anatomical dissection, (II) postmortem histology, and (III) surgical specimen histology were included. Nerve count and area were compared between groups II and III and paravascular sheath thickness between groups I and II. 3D models were generated through 3D histology, nanoCT scanning, and finally through 3D printing. Results A total of 21 specimens were included as follows: Group (I): 5 (3 females, 80–93 years), the SMAP is a complex mesh surrounding the superior mesenteric artery (SMA), branching out, following peripheral arteries and intertwining between them, (II): 7 (5 females, 71–86 years), nerve count: 53 ± 12.42 (38–68), and area: 1.84 ± 0.50 mm2 (1.16–2.29), and (III): 9 (5 females, 55–69 years), nerve count: 31.6 ± 6.74 (range 23–43), and area: 0.889 ± 0.45 mm2 (range 0.479–1.668). SMAP transection injury is 59% of nerve count and 48% of nerve area at middle colic artery origin level. The median values of paravascular sheath thickness decreased caudally from 2.05 to 1.04 mm (anatomical dissection) and from 2.65 to 1.17 mm (postmortem histology). 3D histology models present nerve fibers exclusively within the paravascular sheath, and lymph nodes were observed only outside. NanoCT-derived models reveal oblique nerve fiber trajectories with inclinations between 35° and 55°. Two 3D-printed models of the SMAP were also achieved in a 1:2 scale. Conclusion SMAP surrounds the SMA and branches within the paravascular sheath, while bowel lymph nodes and vessels lie outside. Extent of SMAP injury on histological slides (transection only) was 48% nerve area and 59% nerve count. The 35°–55° inclination range of SMAP nerves possibly imply an even larger injury when plexus excision is performed (lymphadenectomy). Reasons for later improvement of bowel function in these patients can lie in the interarterial nerve fibers between SMA branches.

• Johnsen, Gaute Floer; Haugen, Håvard Jostein; Parreiras Nogueira, Liebert; Sevgi, Usame; Jimenez, Ashley Mae & DeLuca, Joseph T. [Vis alle 8 forfattere av denne artikkelen] (2022). Assessment of the Root Canal Similarity in Contralateral Mandibular Incisors. International Dental Journal. ISSN 0020-6539. doi: 10.1016/j.identj.2022.04.003. Fulltekst i vitenarkiv
• Øvrebø, Øystein; Perale, Giuseppe; Wojciechowski, Jonathan P.; Echalier, Cécile Lucie; Jeffers, Jonathan R. T. & Stevens, Molly M. [Vis alle 8 forfattere av denne artikkelen] (2022). Design and clinical application of injectable hydrogels for musculoskeletal therapy. Bioengineering & Translational Medicine. ISSN 2380-6761. 7(2). doi: 10.1002/btm2.10295. Fulltekst i vitenarkiv Vis sammendrag

  Musculoskeletal defects are an enormous healthcare burden and source of pain and disability for individuals. With an ageing population, the proportion living with these medical indications will increase. Simultaneously, there is pressure on healthcare providers to source efficient solutions, which are cheaper and less invasive than conventional technology. This has led to an increased research focus on hydrogels as highly biocompatible biomaterials that can be delivered through minimally invasive procedures. This review will discuss how hydrogels can be designed for clinical translation, particularly in the context of the new European Medical Device Regulation (MDR). We will then do a deep dive into the clinically used hydrogel solutions that have been commercially approved or have undergone clinical trials in Europe or the US. We will discuss the therapeutic mechanism and limitations of these products. Due to the vast application areas of hydrogels, this work focuses only on treatments of cartilage, bone, and the nucleus pulposus. Lastly, the main steps towards clinical translation of hydrogels as medical devices are outlined. We suggest a framework for how academics can assist small and medium MedTech enterprises conducting the initial clinical investigation and Post-Market Clinical Follow-up (PMCF) required in the MDR. It is evident that the successful translation of hydrogels is governed by acquiring high-quality pre-clinical and clinical data confirming the device mechanism of action and safety.

• Hildebrand, Torben; Parreiras Nogueira, Liebert; Sunde, Pia Titterud; Ørstavik, Dag Solmund; Glasmacher, Birgit & Haugen, Håvard Jostein (2021). Contrast-enhanced nano-CT reveals soft dental tissues and cellular layers. International Endodontic Journal. ISSN 0143-2885. 54(8), s. 1275–1288. doi: 10.1111/iej.13527.
• Rahmati, Maryam; Stoetzel, Sabine; El Khassawna, Thaqif; Mao, Chenyi; Ali, Adilijiang & Vaughan, Joshua C. [Vis alle 14 forfattere av denne artikkelen] (2021). Intrinsically disordered peptides enhance regenerative capacities of bone composite xenografts . Materials Today. ISSN 1369-7021. 52, s. 63–79. doi: 10.1016/j.mattod.2021.12.001. Fulltekst i vitenarkiv Vis sammendrag

  Biomaterial scientists design organic bone substitutes based on the biochemical properties of the mimicked tissue to achieve near native functionality. Several non-collagenous proteins in bone are known as intrinsically disordered proteins (IDPs), as they lack detectible ordered domains and a fixed 3D structure under physiological conditions. Many IDPs perform regulatory roles in a range of cellular functions, which motivated us to design two proline-rich disordered peptides (P2 and P6) and augmented them into the SmartBone® (SBN) biohybrid substitute. Recently we reported an improved proliferation and osteogensis of human osteoblasts and mesenchymal stem cells in the composite groups containing peptides (named here as SBN + P2 and SBN + P6) in vitro. To address the effects of these composites on bone formation and biomineralization, this in vivo study investigated their functions in critical size craniotomy defects in 16 domestic pigs after 8 and 16 weeks of healing. For this purpose, we used cone beam computed tomography (CBCT), microCT (µCT), histology, immunohistochemistry, fluorescent labeling of abundant reactive entities (FLARE), synchrotron SAXS/XRD, optical photothermal IR (O-PTIR) microscopy and nanoscale atomic force microscopy-infrared (AFM-IR) analyses. Our results represent new synthetic IDPs as potential candidates for directing bone formation and biomineralization. The SBN + P6 stimulated significantly higher bone formation and biomineralization after 8 weeks of healing compared to other groups indicating its potential in stimulating early biomineralization. After 16 weeks of healing, the SBN + P2 induced significantly higher bone formation and biomineralization compared to other groups indicating its effects on later bone formation and biomineralization processes. The vigorous stretching of amide primary and secondary IR absorbance peaks at 1660 and 1546 cm−1 in the SBN + P2 group verified that this peptide experienced more conformational changes after 16 weeks of implantation with a higher phosphate intensity at 1037 cm−1 compared to peptide 6. Overall, P2 and P6 are promising candidates for bone augmentation strategies in critical clinical applications. We concluded that FLARE and O-PTIR are promising tools in evaluating and diagnosing the biochemical structure of bone tissue and the bone-biomaterial interface.

• Wang, Zhule; Parreiras Nogueira, Liebert; Haugen, Håvard Jostein; Van Der Geest, Ingrid C M; Caetano de Almeida Ro, Patricia & Janssen, Dennis [Vis alle 9 forfattere av denne artikkelen] (2021). Dual-functional porous and cisplatin-loaded polymethylmethacrylate cement for reconstruction of load-bearing bone defect kills bone tumor cells. Bioactive Materials. ISSN 2452-199X. doi: 10.1016/j.bioactmat.2021.12.023. Fulltekst i vitenarkiv
• Bakkalci, Deniz; Jay, Amrita; Rezaei, Azadeh; Howard, Christopher A.; Haugen, Håvard Jostein & Pape, Judith [Vis alle 12 forfattere av denne artikkelen] (2021). Bioengineering the ameloblastoma tumour to study its efect on bone nodule formation. Scientific Reports. ISSN 2045-2322. 11(24088 ). doi: 10.1038/s41598-021-03484-5. Fulltekst i vitenarkiv Vis sammendrag

  Ameloblastoma is a benign, epithelial cancer of the jawbone, which causes bone resorption and disfigurement to patients affected. The interaction of ameloblastoma with its tumour stroma drives invasion and progression. We used stiff collagen matrices to engineer active bone forming stroma, to probe the interaction of ameloblastoma with its native tumour bone microenvironment. This bone-stroma was assessed by nano-CT, transmission electron microscopy (TEM), Raman spectroscopy and gene analysis. Furthermore, we investigated gene correlation between bone forming 3D bone stroma and ameloblastoma introduced 3D bone stroma. Ameloblastoma cells increased expression of MMP-2 and -9 and RANK temporally in 3D compared to 2D. Our 3D biomimetic model formed bone nodules of an average surface area of 0.1 mm2 and average height of 92.37 ± 7.96 μm over 21 days. We demonstrate a woven bone phenotype with distinct mineral and matrix components and increased expression of bone formation genes in our engineered bone. Introducing ameloblastoma to the bone stroma, completely inhibited bone formation, in a spatially specific manner. Multivariate gene analysis showed that ameloblastoma cells downregulate bone formation genes such as RUNX2. Through the development of a comprehensive bone stroma, we show that an ameloblastoma tumour mass prevents osteoblasts from forming new bone nodules and severely restricted the growth of existing bone nodules. We have identified potential pathways for this inhibition. More critically, we present novel findings on the interaction of stromal osteoblasts with ameloblastoma.

• Thieu, Minh Khai Le; Homayouni, Amin; Hæren, Lena Ringsby; Tiainen, Hanna; Verket, Anders & Ellingsen, Jan Eirik [Vis alle 13 forfattere av denne artikkelen] (2021). Impact of simultaneous placement of implant and block bone graft substitute: an in vivo peri-implant defect model. Biomaterials Research. ISSN 1226-4601. 25(43). doi: 10.1186/s40824-021-00245-3. Fulltekst i vitenarkiv
• Hussain, Badra; Karaca, Ebru Ozkan; Kuru, Bahar Eren; Gursoy, Hare; Haugen, Håvard Jostein & Wohlfahrt, Johan Caspar (2021). Treatment of residual pockets using an oscillating chitosan device versus regular curettes alone—a randomized, feasibility parallel-arm clinical trial. Journal of Periodontology. ISSN 0022-3492. doi: 10.1002/JPER.21-0496. Fulltekst i vitenarkiv
• Hussain, Badra; Haugen, Håvard Jostein; Aass, Anne Merete; Sanz Alonso, Mariano; Antonoglou, Georgios & Bouchard, Philippe [Vis alle 23 forfattere av denne artikkelen] (2021). Peri-Implant Health and the Knowing-Doing Gap—A Digital Survey on Procedures and Therapies. Frontiers in Dental Medicine. ISSN 2673-4915. 2(726607). doi: 10.3389/fdmed.2021.726607. Fulltekst i vitenarkiv
• Rahmati, Maryam; Stötzel, Sabine; El Khassawna, Thaqif; Iskhahova, Kamila; Wieland, D.C. Florian & Plumhoff, Berit Zeller [Vis alle 7 forfattere av denne artikkelen] (2021). Early Osteoimmunomodulatory Effects of Magnesium–Calcium–Zinc alloys. Journal of Tissue Engineering. ISSN 2041-7314. doi: 10.1177/20417314211047100. Fulltekst i vitenarkiv Vis sammendrag

  Today, substantial attention is given to biomaterial strategies for bone regeneration, and among them, there is a growing interest in using immunomodulatory biomaterials. The ability of a biomaterial to induce neo vascularization and macrophage polarization is a major factor in defining its success. Magnesium (Mg)-based degradable alloys have attracted significant attention for bone regeneration owing to their biodegradability and potential for avoiding secondary removal surgeries. However, there is insufficient evidence in the literature regarding the early inflammatory responses to these alloys in vivo. In this study, we investigated the early body responses to Mg 0.45 wt%Zn-0.45 wt%Ca pin-shaped alloy (known as ZX00 alloy) in rat femora 2, 5 and 10 days after implantation. We used 3D micro computed tomography (μCT), histological, immunohistochemical, histomorphometrical and small angle X-ray scattering (SAXS) analyses to study new bone formation, early macrophage polarization, neo vascularization and bone quality at the implant bone interface. The expression of macrophage type 2 biological markers increased significantly after 10 days of Mg alloy implantation, indicating its potential in stimulating macrophage polarization. Our biomineralization results using μCT as well as histological stained sections did not indicate any statistically significant differences between different time points for both groups. The activity of alkaline phosphatase (ALP) and Runt-related transcription factor 2 (Runx 2) biological markers decreased significantly for Mg group, indicating less osteoblast activity. Generally, our results supported the potential of ZX00 alloy to enhance the expression of macrophage polarization in vivo; however, we could not observe any statistically significant changes regarding biomineralization.

• Palombo, David; Rahmati, Maryam; Vignoletti, Fabio; Esporrin, Javier Sanz; Haugen, Håvard Jostein & Sanz, Mariano (2021). Hard and soft tissue healing around implants with a modified implant neck configuration: an experimental in vivo preclinical investigation. Clinical Oral Implants Research. ISSN 0905-7161. doi: 10.1111/clr.13812. Fulltekst i vitenarkiv Vis sammendrag

  Objectives: To evaluate the dimensions and morphology of the peri-implant tissues around a modified dental implant designed with a tissue level connection and a convergent transmucosal neck.This test implant was compared with a conventional bone level implant connected to a cylindrical machined titanium abutment. Material and methods: Eight experimental animals were used for this in vivo investigation, where 16 test and 16 control implants were placed following a random allocation sequence. The following histological outcomes were evaluated 4 and 12 weeks after implantation: the morphology of peri-implant tissues, the soft tissue height and thickness, the horizontal and vertical bone remodeling and the bone to implant contact (BIC). Results: In both early (4 weeks) and late (12 weeks) healing times, there were no statistically significant differences between test and control implants, with respect to the overall height and thickness of the peri-implant hard and soft tissues. There was a tendency toward a more coronal free gingival margin (I-FGM) at the buccal aspect of test when compared to control implants (at 4 weeks, difference of 0.97mm (p=0.572) and 0.30mm (p=1.000) at 12 weeks). Similarly, there was a tendency toward a more coronal position of the first bone to implant contact (I-B) at the buccal aspect of test as compared to control implants (1.08mm (p=0.174) at 4 weeks and 0.83mm (p=0.724) at 12 weeks).

• Marovic, Danijela; Haugen, Håvard Jostein; Mandic, Visnja Negovetic; Par, Matej; Zheng, Kai & Tarle, Zrinka [Vis alle 7 forfattere av denne artikkelen] (2021). Incorporation of Copper-Doped Mesoporous Bioactive Glass Nanospheres in Experimental Dental Composites: Chemical and Mechanical Characterization. Materials. ISSN 1996-1944. 14(2611), s. 1–12. doi: 10.3390/ma14102611. Fulltekst i vitenarkiv Vis sammendrag

  Experimental dental resin composites incorporating copper-doped mesoporous bioactive glass nanospheres (Cu-MBGN) were designed to impart antibacterial and remineralizing properties. The study evaluated the influence of Cu-MBGN on the mechanical properties and photopolymerization of resin composites. Cu-MBGN were synthesized using a microemulsion-assisted sol–gel method. Increasing amounts of Cu-MBGN (0, 1, 5, and 10 wt %) were added to the organic polymer matrix with inert glass micro- and nanofillers while maintaining a constant resin/filler ratio. Six tests were performed: X-ray diffraction, scanning electron microscopy, flexural strength (FS), flexural modulus (FM), Vickers microhardness (MH), and degree of conversion (DC). FS and MH of Cu-MBGN composites with silica fillers showed no deterioration with aging, with statistically similar results at 1 and 28 days. FM was not influenced by the addition of Cu-MBGN but was reduced for all tested materials after 28 days. The specimens with 1 and 5% Cu-MBGN had the highest FS, FM, MH, and DC values at 28 days, while controls with 45S5 bioactive glass had the lowest FM, FS, and MH. DC was high for all materials (83.7–93.0%). Cu-MBGN composites with silica have a potential for clinical implementation due to high DC and good mechanical properties with adequate resistance to aging.

• D. Tien, Nguyen; Lyngstadaas, Ståle Petter; Mano, Joao F.; Blaker, Jonathan James & Haugen, Håvard Jostein (2021). Recent Developments in Chitosan-Based Micro/Nanofibers for Sustainable Food Packaging, Smart Textiles, Cosmeceuticals, and Biomedical Applications. Molecules. ISSN 1431-5157. 26(2683), s. 1–24. doi: 10.3390/molecules26092683. Fulltekst i vitenarkiv Vis sammendrag

  Chitosan has many useful intrinsic properties (e.g., non-toxicity, antibacterial properties, and biodegradability) and can be processed into high-surface-area nanofiber constructs for a broad range of sustainable research and commercial applications. These nanofibers can be further functionalized with bioactive agents. In the food industry, for example, edible films can be formed from chitosan-based composite fibers filled with nanoparticles, exhibiting excellent antioxidant and antimicrobial properties for a variety of products. Processing ‘pure’ chitosan into nanofibers can be challenging due to its cationic nature and high crystallinity; therefore, chitosan is often modified or blended with other materials to improve its processability and tailor its performance to specific needs. Chitosan can be blended with a variety of natural and synthetic polymers and processed into fibers while maintaining many of its intrinsic properties that are important for textile, cosmeceutical, and biomedical applications. The abundance of amine groups in the chemical structure of chitosan allows for facile modification (e.g., into soluble derivatives) and the binding of negatively charged domains. In particular, high-surface-area chitosan nanofibers are effective in binding negatively charged biomolecules. Recent developments of chitosan-based nanofibers with biological activities for various applications in biomedical, food packaging, and textiles are discussed herein.

• Torben, Hildebrand; Nogueira, Liebert Parreiras; Sunde, Pia Titterud; Ørstavik, Dag; Glasmacher, Birgit & Haugen, Håvard Jostein (2021). Contrast-enhanced nano-CT reveals dental soft tissues and cellular layers. International Endodontic Journal. ISSN 0143-2885. doi: 10.1111/iej.13527. Fulltekst i vitenarkiv Vis sammendrag

  Aim: Nano-CT technologies offer 3D imaging methods that allow high-resolution examination of bones and teeth, but soft tissue components have weak X-ray attenuation and are not easily visualised in CT images. We introduce a methodology designed to simultaneously visualise dental ultrastructure, including cellular and soft tissue components, by utilising phosphotungstic acid (PTA) as a contrast-enhancement agent. Methodology: Sound third molars were collected from healthy human adults and fixed in 4% buffered paraformaldehyde. To evaluate the impact of PTA in concentrations of 0.3%, 0.7% and 1% on dental soft and hard tissues for CT imaging, cementum and dentine-pulp sections were cut, dehydrated and stained with immersion periods of 12 hours, 24 hours, 2 days or 5 days. The samples were scanned with high-resolution nano-CT, where we examined both the cementum and pulpal regions with pixel sizes down to 0.5 µm for dental-pulp sections. Results: Dental cementum and periodontium as well as odontoblasts and predentine were made visible through PTA-staining in high-resolution three-dimensional nano-CT scans. Different segments of the tooth required different staining protocols. The thickness of the cementum could be computed over the height of the tooth once it was made visible by the PTA-enhanced contrast, and the attached soft tissue components of the interior of the tooth could be shown on the dentine-pulp interface in greater detail. Three-dimensional illustrations allowed a histology-like visualisation of the sections in all orientations with a single scan and easy sample preparation. The segmentation of the sigmoidal dentinal tubules and the surrounding dentine allowed a three-dimensional investigation and quantative of the dentine composition, such as the tubular lumen or the ratio of the tubular lumen area to the dentinal surface. Conclusion: The staining protocol made it possible to visualise hard tissues along with cellular layers and soft tissues using a laboratory-based nano-CT technique. The protocol depended on both tissue type and size. This methodology offers enhanced possibilities for the concomitant visualisation of soft and hard dental tissues.

• Zhu, Hao; Haugen, Håvard Jostein; Perale, Giuseppe; Reseland, Janne Elin; Nogueira, Liebert Parreiras & Cantalapiedra, Antonio gonzalez [Vis alle 11 forfattere av denne artikkelen] (2021). Tailoring Resorption Rates and Osteogenic Response in Xeno-Hybrid Bone Grafts: The Effect of Added Gelatins. Engineering (Beijing). ISSN 2095-8099. 13, s. 197–208. doi: 10.1016/j.eng.2021.01.010. Fulltekst i vitenarkiv
• Thieu, Minh Khai Le; Haugen, Håvard Jostein; Esporrin, Javier Sanz; Sanz, Mariano; Lyngstadaas, Ståle Petter & Verket, Anders (2021). Guided bone regeneration of chronic non-contained bone defects using a volume stable porous block TiO2 scaffold: An experimental in vivo study. Clinical Oral Implants Research. ISSN 0905-7161. doi: 10.1111/clr.13708. Vis sammendrag

  Objectives To evaluate new lateral bone formation and lateral volume augmentation by guided bone regeneration (GBR) in chronic non‐contained bone defects with the use of a non‐resorbable TiO2‐block. Materials and methods Three buccal bone defects were created in each hemimandible of eight beagle dogs and allowed to heal for eight weeks before treatment by GBR. Each hemimandible was randomly allocated to 4 or 12‐week healing time after GBR and three intervention groups were assigned by block randomization: TiO2 block: TiO2‐scaffold and a collagen membrane, DBBM particles: Deproteinized bovine bone mineral (DBBM) and a collagen membrane, Empty control: Collagen membrane only. Microcomputed tomography (microCT) was used to measure the lateral bone formation and width augmentation. Histological outcomes included descriptive analysis and histomorphometric measurements. Results MicroCT analysis demonstrated increasing new bone formation from four to twelve weeks of healing. The greatest width of mineralized bone was seen in the empty controls, and the largest lateral volume augmentation was observed in the TiO2 block sites. The DBBM particles demonstrated more mineralized bone in the grafted area than the TiO2 blocks, but small amounts and less than the empty control sites. Conclusion The TiO2 blocks rendered the largest lateral volume augmentation but also less new bone formation compared to the DBBM particles. The most new lateral bone formation outward from the bone defect margins was observed in the empty controls, indicating that the presence of either graft material leads to slow appositional bone growth.

• Di Raimondo, Ricardo; Sanz, Javier; Sanz Martin1, Ignacio; Vignoletti, Fabio; Nuñez, Javi & Muñoz, Fernando [Vis alle 8 forfattere av denne artikkelen] (2021). Hard tissue volumetric and soft tissue contour linear changes at implants with different surface characteristics after experimentally induced peri-implantitis: an experimental in vivo investigation. Clinical Oral Investigations. ISSN 1432-6981. doi: 10.1007/s00784-020-03720-8. Vis sammendrag

  Objective To evaluate the hard tissue volumetric and soft tissue contour linear changes in implants with two different implant surface characteristics after a ligature-induced peri-implantitis. Material andmethods In eight beagle dogs, implants with the same size and diameter but distinct surface characteristics were placed in the healed mandibular sites. Test implants had an external monolayer of multi-phosphonate molecules (B+), while control implants were identical but without the phosphonate-rich surface. Once the implants were osseointegrated, oral hygiene was interrupted and peri-implantitis was induced by placing subgingival ligatures. After 16 weeks, the ligatures were removed and peri-implantitis progressed spontaneously. Bone to implant contact (BIC) and bone loss (BL) were assessed three-dimensionally with Micro-Ct (μCT). Dental casts were optically scanned and the obtained digitalized standard tessellation language (STL) images were used to assess the soft tissue vertical and horizontal contour linear changes. Results Reduction of the three-dimensional BIC percentage during the induction and progression phases of the experimental peri-implantitis was similar for both the experimental and control implants, without statistically significant differences between them. Soft tissue analysis revealed for both implant groups an increase in horizontal dimension after the induction of peri-implantitis, followed by a decrease after the spontaneous progression period. In the vertical dimension, a soft tissue dehiscence was observed in both groups, being more pronounced at the buccal aspect. Conclusions The added phosphonate-rich surface did not provide a more resistant environment against experimental periimplantitis, when assessed by the changes in bone volume and soft tissue contours. Clinical relevance Ligature-induced peri-implantitis is a validated model to study the tissue changes occurring during peri-implantitis. It was hypothesized that a stronger osseointegration mediated by the chemical bond of a phosphonaterich implant surface would develop an environment more resistant to the inflammatory changes occurring after experimental peri-implantitis. These results, however, indicate that the hard and soft tissue destructive changes occurring at both the induction and progression phases of experimental peri-implantitis were not influenced by the quality of osseointegration.

• Vyas, Cian; Zhang, Jun; Øvrebø, Øystein; Huang, Boyang; Roberts, Iwan & Setty, Mohan [Vis alle 10 forfattere av denne artikkelen] (2021). 3D printing of silk microparticle reinforced polycaprolactone scaffolds for tissue engineering applications. Materials Science and Engineering C: Materials for Biological Applications. ISSN 0928-4931. 118. doi: 10.1016/j.msec.2020.111433. Fulltekst i vitenarkiv Vis sammendrag

  Polycaprolactone (PCL) scaffolds have been widely investigated for tissue engineering applications, however, they exhibit poor cell adhesion and mechanical properties. Subsequently, PCL composites have been produced to improve the material properties. This study utilises a natural material, Bombyx mori silk microparticles (SMP) prepared by milling silk fibre, to produce a composite to enhance the scaffolds properties. Silk is biocompatible and biodegradable with excellent mechanical properties. However, there are no studies using SMPs as a reinforcing agent in a 3D printed thermoplastic polymer scaffold. PCL/SMP (10, 20, 30 wt. %) composites were prepared by melt blending. Rheological analysis showed that SMP loading increased the shear thinning and storage modulus of the material. Scaffolds were fabricated using a screw-assisted extrusion-based additive manufacturing system. Scanning electron microscopy and x-ray microtomography was used to determine scaffold morphology. The scaffolds had high interconnectivity with regular printed fibres and pore morphologies within the designed parameters. Compressive mechanical testing showed that the addition of SMP significantly improved the compressive Young’s modulus of the scaffolds. The scaffolds were more hydrophobic with the inclusion of SMP which was linked to a decrease in total protein adsorption. Cell behaviour was assessed using human adipose derived mesenchymal stem cells. A cytotoxic effect was observed at higher particle loading (30 wt. %) after 7 days of culture. By day 21, 10 wt. % loading showed significantly higher cell metabolic activity and proliferation, high cell viability, and cell migration throughout the scaffold. Calcium mineral deposition was observed on the scaffolds during cell culture. Large calcium mineral deposits were observed at 30 wt. % and smaller calcium deposits were observed at 10 wt. %. This study demonstrates that SMPs incorporated into a PCL scaffold provided effective mechanical reinforcement, improved the rate of degradation, and increased cell proliferation, demonstrating potential suitability for bone tissue engineering applications.

• Sukul, Mousumi; Sahariah, Priyanka; Lauzon, Hélène L.; Borges, João; Másson, Már & Mano, Joao F. [Vis alle 8 forfattere av denne artikkelen] (2021). In vitro biological response of human osteoblasts in 3D chitosan sponges with controlled degree of deacetylation and molecular weight. Carbohydrate Polymers. ISSN 0144-8617. 254. doi: 10.1016/j.carbpol.2020.117434. Fulltekst i vitenarkiv Vis sammendrag

  We have studied the effect of chitosan sponges, produced from chitosan batches with distinct degree of deacetylation (DDA) and molecular weight (Mw), on the adhesion, growth and differentiation of primary human osteoblasts with an aim to offer a suitable tool for guided bone regeneration. All the chitosan sponges revealed similar microstructure, irrespective of the DDA (58, 73, 82, 88, and 91 %) and Mw (749, 547, 263, 215, and 170 kDa, respectively). Cell spreading was higher on sponges having a higher DDA. Higher DDA induced a more pronounced increase in alkaline phosphatase activity, osteopontin (OPN), vascular endothelial growth factor-A (VEGF), interleukin-6 (IL-6), and reduction in monocyte chemoattractant protein-1 (MCP-1), sclerostin (SOST) and dickkopf related protein-1 as compared to lower DDA. Lower DDA induced the increased secretion of osteoprotegerin and SOST as compared to higher DDA. The combination of higher DDA and Mw induced an increased secretion of VEGF and IL-6, however reduced the secretion of OPN as compared to chitosan with similar DDA but with lower Mw. In summary, the variations in cellular responses to the different chitosan sponges indicate a potential for individual tailoring of desired responses in guided

• Zhu, Hao; Blahnová, Veronika Hefka; Perale, Giuseppe; Xiao, Jun; Betge, Felice & Boniolo, Fabio [Vis alle 9 forfattere av denne artikkelen] (2020). Xeno-Hybrid Bone Graft Releasing Biomimetic Proteins Promotes Osteogenic Differentiation of hMSCs. Frontiers in Cell and Developmental Biology. ISSN 2296-634X. doi: 10.3389/fcell.2020.619111. Fulltekst i vitenarkiv
• Kallas, Pawel; Haugen, Håvard Jostein; Gadegaard, Nikolaj; Stormonth-Darling, John; Hulander, Mats & Andersson, Martin [Vis alle 7 forfattere av denne artikkelen] (2020). Adhesion of Escherichia Coli to Nanostructured Surfaces and the Role of Type 1 Fimbriae. Nanomaterials. ISSN 2079-4991. 10(11). doi: 10.3390/nano10112247. Fulltekst i vitenarkiv Vis sammendrag

  Bacterial fimbriae are an important virulence factor mediating adhesion to both biotic and abiotic surfaces and facilitating biofilm formation. The expression of type 1 fimbriae of Escherichia coli is a key virulence factor for urinary tract infections and catheter-associated urinary tract infections, which represent the most common nosocomial infections. New strategies to reduce adhesion of bacteria to surfaces is therefore warranted. The aim of the present study was to investigate how surfaces with different nanotopography-influenced fimbriae-mediated adhesion. Surfaces with three different nanopattern surface coverages made in polycarbonate were fabricated by injection molding from electron beam lithography nanopatterned templates. The surfaces were constructed with features of approximately 40 nm width and 25 nm height with 100 nm, 250 nm, and 500 nm interspace distance, respectively. The role of fimbriae type 1-mediated adhesion was investigated using the E. coli wild type BW25113 and ΔfimA (with a knockout of major pilus protein FimA) and ΔfimH (with a knockout of minor protein FimH) mutants. For the surfaces with nanotopography, all strains adhered least to areas with the largest interpillar distance (500 nm). For the E. coli wild type, no difference in adhesion between surfaces without pillars and the largest interpillar distance was observed. For the deletion mutants, increased adhesion was observed for surfaces without pillars compared to surfaces with the largest interpillar distance. The presence of a fully functional type 1 fimbria decreased the bacterial adhesion to the nanopatterned surfaces in comparison to the mutants.

• Gross, Karlis Agris; Petzold, Christiane; Pluduma, Liene; Kumermanis, Maris & Haugen, Håvard Jostein (2020). Structural and Chemical Hierarchy in Hydroxyapatite Coatings . Materials. ISSN 1996-1944. 13(19), s. 1–15. doi: 10.3390/ma13194447. Fulltekst i vitenarkiv Vis sammendrag

  Hydroxyapatite coatings need similarly shaped splats as building blocks and then a homogeneous microstructure to unravel the structural and chemical hierarchy for more refined improvements to implant surfaces. Coatings were thermally sprayed with differently sized powders (20-40, 40-63 and 63-80 µm) to produce flattened homogeneous splats. The surface was characterized for splat shape by profilometry and AFM, crystal size by AFM, crystal orientation by X-ray diffraction (XRD) and structural variations by XRD. Chemical composition was assessed by phase analysis, but variations in chemistry were detected by XRD and Raman spectroscopy. The resulting surface electrical potential was measured by Kelvin probe AFM. Five levels of structural hierarchy were suggested: the coating, the splat, oriented crystals, alternate layers of oxyapatite and HAp, and the suggested anion orientation. Chemical hierarchy was present over a lower range of order for smaller splats. Coatings made from smaller splats exhibited a greater electrical potential, inferred to arise from oxyapatite, and supplemented by ordered OH- ions in a rehydroxylated surface layer. A model has been proposed to show the influence of structural hierarchy on the electrical surface potential. Structural hierarchy is proposed as a means to further refine the properties of implant surfaces.

Se alle arbeider i Cristin

• Hussain, Badra; Simm, Roger; Bueno, Jaime; Giannettou, Savvas; Naemi, Ali-Oddin & Lyngstadaas, Ståle Petter [Vis alle 7 forfattere av denne artikkelen] (2023). Chemical decontamination strategies for treatment of peri-implant disease.
• Haugen, Håvard Jostein; Parreiras Nogueira, Liebert & Hildebrand, Torben (2023). Novel techniques to visualise cellular tissues using X-ray imaging.
• Haugen, Håvard Jostein (2023). Decontamination of infected dental implants.
• Reese, Jan-Ole; Haugen, Håvard Jostein; Saragliadis, Athanasios & Linke, Dirk (2023). Development of multi-species biofilm models for evaluating medical approaches in dentistry .
• Hussain, Badra; Simm, Roger; Lyngstadaas, Ståle Petter; Bueno, Jamie & Haugen, Håvard Jostein (2023). Chemical decontamination strategies for treatment of peri-implant disease: using an in vitro dental pellicle-, and a multispecies biofilm model.
• Reese, Jan-Ole; Saragliadis, Athanasios; Haugen, Håvard Jostein & Linke, Dirk (2023). Development of multi-species biofilm models for evaluating medical approaches in dentistry.
• Alam, Anam; Haugen, Håvard Jostein; Ma, Qianli & Loča, Dagnija (2023). USING EGGSHELLS FOR BONE GRAFTS. Springwise. Vis sammendrag

  Bone grafts are considered to be the gold standard in terms of repairing and reconstructing damaged bones. These can either be autologous, where a patient’s own tissue is used, or allogeneic, where the tissue is taken from another person. But limited supply, donor-site complications, and risk of disease transmission can often prevent allogeneic grafts from being used. Xenogeneic bone grafts, where the graft is taken from another species, represent a feasible alternative. But, because xenograft materials come from mammal tissue, it raises ethical questions surrounding animal welfare. To find a safe, adaptable, and environmentally friendly alternative, a team of researchers has developed a method to convert eggshells into endotoxin-free and immunocompatible amorphous calcium phosphate (ACP) particles. First the shells were heated, then mixed with distilled water and phosphoric acid. The precipitate was then filtered and washed, before being submerged in liquid nitrogen. The resulting ACP particles are essential to forming hard and strong bone and can therefore be used as an ideal bone substitute. As well as containing plenty of calcium and phosphorous, Dr Qianli Ma – the study’s lead author – highlights that eggshells are also an ideal raw material because they contain traces of magnesium and strontium, which are associated with healthy bone regeneration. The team also created a novel 3D spheroid model, which allows the activity of eggshell ACP to be studied in vitro. With the model, the researchers could observe how the ACP materials would actually interact with osteoblasts, and they were found to be safe and effective in promoting bone regeneration. If scaled successfully, this new technique could enable an unlimited supply of sustainable bone graft materials, while reducing the volume of eggshells going to waste. The scientists hope their latest findings inspire additional research into the conversion of food waste into high-value biomaterials. Eggshells are not only being used in the medical field. Springwise has also spotted a design studio creating wall tiles from discarded eggshells, and another that has transformed the waste into car interiors

• Haugen, Håvard Jostein (2023). How to clean infected implants.
• Reese, Jan-Ole; Saragliadis, Athanasios; Haugen, Håvard Jostein & Linke, Dirk (2023). Development of multi-species biofilm models for testing biotechnological approaches in dentistry.
• Bota, Maria; Tarle, Zrinka; Par, Matej; Haugen, Håvard Jostein; Zheng, Kai & Boccaccini, Aldo R [Vis alle 7 forfattere av denne artikkelen] (2022). Crosslinking density of experimental dental composites with copper-doped mesoporous bioactive nanospheres. Acta Stomatologica Croatica. ISSN 0001-7019. 56(4). doi: 10.15644/asc56/4/6.
• Haugen, Håvard Jostein (2022). Bone tissue engineering.
• Haugen, Håvard Jostein (2022). Additive Manufacturing for Tissue Engineering and Regenerative Medicine – Grand Challenges.
• Haugen, Håvard Jostein (2022). Advances in biomaterials engineering: Development of biomaterials, in vitro and in vivo models and investigations, drug delivery systems, implant manufacturing processes, 3D printing, and applications in the clinic.
• Haugen, Håvard Jostein (2022). Scaffolds for infected bone sites.
• Reese, Jan-Ole; Saragliadis, Athanasios; Haugen, Håvard Jostein & Linke, Dirk (2022). Development of multi-species biofilm models for testing biotechnological approaches in dentistry.
• Zhu, Hao; Ji, Xiongfa; Guan, Hanfeng; Zhao, Liming; Zhao, Libo & Liu, Changyu [Vis alle 12 forfattere av denne artikkelen] (2021). Corrigendum to “Tantalum nanoparticles reinforced polyetheretherketone shows enhanced bone formation” [Mater. Sci. Eng. C 101 (2019) 232–242] (Materials Science & Engineering C (2019) 101 (232–242), (S0928493118321453), (10.1016/j.msec.2019.03.091)). Materials Science and Engineering C: Materials for Biological Applications. ISSN 0928-4931. 128. doi: 10.1016/j.msec.2021.112332.
• Abadpour, Shadab; Niemi, Essi Maria; Strid Orrhult, Linnea; Parreiras Nogueira, Liebert; Haugen, Håvard Jostein & Josefsen, Dag [Vis alle 10 forfattere av denne artikkelen] (2021). 307.7: 3D Bioprinting of Functional Islets With Adipose-derived Stromal Cells in an Alginate/Nanocellulose Scaffold. Transplantation. ISSN 0041-1337. s. S25–S25. doi: 10.1097/01.tp.0000804424.50335.7e.
• Haugen, Håvard Jostein & Engqvist, Håkan (2021). first editorial for the new journal Scandinavian Journal of Materials Science. Scandinavian Journal of Materials Science (ScandJMS). ISSN 2004-2124. 1(1). doi: 10.2340/scandjms.v1.883.
• Rahmati, Maryam; Johannes Frank, Matthias; Martin Walter, Sebastian; Cabrer Monjo, Marta; Satué, Maria & Reseland, Janne Elin [Vis alle 8 forfattere av denne artikkelen] (2021). Osteo-immune responses to the enamel matrix derivative coating layer on titanium-based implants .
• Rahmati, Maryam; Palombo, David; Vignoletti, Fabio; Javier, Sanz-Esporrin; Haugen, Håvard Jostein & Sanz, Mariano (2021). Tissue healing around PRAMA vs. PREMIUM implants .
• Johannes Frank, Matthias; Walter, Martin Sebastian; Cabrer Monjo, Marta; Satué, Maria; Reseland, Janne E & Ståle Petter, Lyngstadaas [Vis alle 7 forfattere av denne artikkelen] (2021). Osteo-immune responses to the enamel matrix derivative coating layer on titanium-based implants .
• Haugen, Håvard Jostein & Parreiras Nogueira, Liebert (2021). Novel contrast agent for visualisation of soft tissue with X-ray imaging.
• Haugen, Håvard Jostein; Parreiras Nogueira, Liebert & Sunde, Pia Titterud (2021). Use of novel contrast enhanced agent for tissue engineering.
• Haugen, Håvard Jostein; Lyngstadaas, Ståle Petter; Monjo Cabrer, Marta & Perale, Giuseppe (2021). XENO-HYBRID BONE GRAFT RELEASING MATRIX-DERIVED INTRINSICALLY DISORDERED PROTEINS PROMOTES OSTEOGENIC DIFFERENTIATION OF HMSCS.
• Haugen, Håvard Jostein; Thieu, Minh Khai Le; Sanz-Esporrin, Javier; Sanz Alonso, Mariano & Verket, Anders (2021). GUIDED BONE REGENERATION OF CHRONIC NON-CONTAINED BONE DEFECTS USING A VOLUME STABLE POROUS TIO2 SCAFFOLD: AN EXPERIMENTAL IN VIVO STUDY .
• Linke, Dirk; Økstad, Ole Andreas; Haugen, Håvard Jostein & Winther-Larsen, Hanne Cecilie (2021). The exchange program MISC - Options for internships at the University of Oslo (with descriptions of current projects).
• Rahmati, Maryam; Stoetzel, Sabine; El Khassawna, Thaqif; Wieland, D.C. Florian; Plumhoff, Berit Zeller & Iskhahova, Kamila [Vis alle 7 forfattere av denne artikkelen] (2021). 2&3D imaging of osteogenesis and macrophage polarization in magnesium-based alloys .
• Rahmati, Maryam; Stoetzel, Sabine; El Khassawna, Thaqif; Wieland, Florian; Iskhahova, Kamila & Plumhoff, Berit Zeller [Vis alle 7 forfattere av denne artikkelen] (2021). EARLY INFLAMMATORY RESPONSES TO MAGNESIUM-BASED ALLOYS.
• Haugen, Håvard Jostein; Hussain, Badra & Bjørkeng, Astrid Skiftesvik (2021). Er biomaterialer kroppens indre plaster? [Internett]. Universitetsplassen Podcast. Vis sammendrag

  Ting går i stykker. Også i kroppen. Men medisinsk forskning har gjort det mulig å erstatte noen ødelagte deler med biomaterialer. I denne podkasten forklarer forskerne Håvard Haugen og Badra Hussein hva biomaterialer er og hvordan de brukes - og ikke minst hvordan de forsker på nye biomaterialer for å lage nytt bein.

• Farè, Silvia; Torricelli, Paola; Giavaresi, Gianluca; Bertoldi, Serena; Alessandrino, Antonio & Villa, Tomaso [Vis alle 10 forfattere av denne artikkelen] (2020). Corrigendum to “In vitro study on silk fibroin textile structure for Anterior Cruciate Ligament regeneration” [Mater. Sci. Eng. C (2013) 3601–360] (Materials Science and Engineering C, (S0928493113002415), (10.1016/j.msec.2013.04.027)). Materials Science and Engineering C: Materials for Biological Applications. ISSN 0928-4931. 116. doi: 10.1016/j.msec.2020.111193.

Se alle arbeider i Cristin