Hanna Tiainen

• Rogala, Agnes; Zaytseva-Zotova, Daria; Oreja Remartinez, Enrique; Barrantes Bautista, Alejandro & Tiainen, Hanna (2024). Combining QCM-D with live-cell imaging reveals the impact of serum proteins on the dynamics of fibroblast adhesion on tannic acid-functionalised surfaces. Biomaterials Science. ISSN 2047-4830. 12, s. 3345– 3359. doi: 10.1039/d4bm00184b. Fulltekst i vitenarkiv
• Khan, Sadia Nazir; Koldsland, Odd Carsten; Tiainen, Hanna & Hjortsjö, Carl (2024). Anatomical three-dimensional model with peri-implant defect for in vitro assessment of dental implant decontamination. Clinical and Experimental Dental Research. ISSN 2057-4347. 10(1). doi: 10.1002/cre2.841. Fulltekst i vitenarkiv
• Munir, Arooj; Reseland, Janne Elin; Tiainen, Hanna; Haugen, Håvard Jostein; Sikorski, Pawel & Christiansen, Emil [Vis alle 9 forfattere av denne artikkelen] (2023). Osteocyte-Like Cells Differentiated From Primary Osteoblasts in an Artificial Human Bone Tissue Model. JBMR Plus. ISSN 2473-4039. s. 1–10. doi: 10.1002/jbm4.10792. Fulltekst i vitenarkiv
• Printzell, Lisa; Reseland, Janne Elin; Edin, Nina Frederike Jeppesen; Ellingsen, Jan Eirik & Tiainen, Hanna (2023). Backscatter from therapeutic doses of ionizing irradiation does not impair cell migration on titanium implants in vitro. Clinical Oral Investigations. ISSN 1432-6981. doi: 10.1007/s00784-023-05128-6. Fulltekst i vitenarkiv
• Printzell, Lisa; Reseland, Janne Elin; Edin, Nina Frederike Jeppesen; Tiainen, Hanna & Ellingsen, Jan Eirik (2023). The dose-dependent impact of γ-radiation reinforced with backscatter from titanium on primary human osteoblasts. Biomaterial Investigations in Dentistry. 10(1). doi: 10.1080/26415275.2023.2209116. Fulltekst i vitenarkiv
• Spoutil, Frantisek; Aranaz-Novaliches, Goretti; Prochazkova, Michaela; Wald, Tomas; Novosadova, Vendula & Kasparek, Petr [Vis alle 14 forfattere av denne artikkelen] (2023). Early evolution of enamel matrix proteins is reflected by pleiotropy of physiological functions. Scientific Reports. ISSN 2045-2322. 13(1). doi: 10.1038/s41598-023-28388-4. Fulltekst i vitenarkiv
• Fontelo, Raul; Soares da Costa, Diana; Gomez-Florit, Manuel; Tiainen, Hanna; Reis, Rui L. & Novoa-Carballal, Ramon [Vis alle 7 forfattere av denne artikkelen] (2022). Antibacterial nanopatterned coatings for dental implants. Journal of materials chemistry. B. ISSN 2050-750X. doi: 10.1039/d2tb01352e. Fulltekst i vitenarkiv
• Weber, Florian; Morais Dornelas Figueira, Louise; Hafiane, Nora; Zaytseva-Zotova, Daria; Barrantes Bautista, Alejandro & Petersen, Fernanda Cristina [Vis alle 7 forfattere av denne artikkelen] (2022). Can polyphenolic surface modifications prevent fungal colonization of titanium dental implants? Colloids and Surfaces B: Biointerfaces. ISSN 0927-7765. 219. doi: 10.1016/j.colsurfb.2022.112813. Fulltekst i vitenarkiv
• Weber, Florian; Quach, Huy Quang; Reiersen, Mathias; Sarraj, Sadaf Yosef; Bakir, Dyala Nidal Akram & Jankowski, Victor Aleksander [Vis alle 8 forfattere av denne artikkelen] (2022). Characterization of the foreign body response of titanium implants modified with polyphenolic coatings. Journal of Biomedical Materials Research. Part A. ISSN 1549-3296. doi: 10.1002/jbm.a.37377. Fulltekst i vitenarkiv
• Thieu, Minh Khai Le; Homayouni, Amin; Hæren, Lena Ringsby; Tiainen, Hanna; Verket, Anders & Ellingsen, Jan Eirik [Vis alle 13 forfattere av denne artikkelen] (2021). Impact of simultaneous placement of implant and block bone graft substitute: an in vivo peri-implant defect model. Biomaterials Research. ISSN 1226-4601. 25(43). doi: 10.1186/s40824-021-00245-3. Fulltekst i vitenarkiv
• Chahal, Aman S; Gomez-Florit, Manuel; Domingues, Rui M. A,; Gomes, Manuela E. & Tiainen, Hanna (2021). Human platelet lysate-loaded poly(ethylene glycol) hydrogels induce stem cell chemotaxis in vitro. Biomacromolecules. ISSN 1525-7797. doi: 10.1021/acs.biomac.1c00573. Fulltekst i vitenarkiv
• Klemm, Anne & Tiainen, Hanna (2021). Highly porous Sr-doped TiO2 ceramics maintain compressive strength after grain boundary corrosion. Journal of the European Ceramic Society. ISSN 0955-2219. doi: 10.1016/j.jeurceramsoc.2021.04.059. Fulltekst i vitenarkiv
• Vilaça, Adriana; Domingues, Rui M. A,; Tiainen, Hanna; Mendes, Bárbara B.; Barrantes Bautista, Alejandro & Reis, Rui L. [Vis alle 8 forfattere av denne artikkelen] (2021). Multifunctional surfaces for improving soft tissue integration. Advanced Healthcare Materials. ISSN 2192-2640. doi: 10.1002/adhm.202001985. Fulltekst i vitenarkiv
• Hugo, Jørgen; Koldsland, Odd Carsten; Aass, Anne Merete & Tiainen, Hanna (2020). Development and initial testing of an in vitro model simulating class II furcation defects. Clinical and Experimental Dental Research. ISSN 2057-4347. doi: 10.1002/cre2.346. Fulltekst i vitenarkiv
• Weber, Florian; Sagstuen, Einar; Zhong, Qi-Zhi; Zheng, Tian & Tiainen, Hanna (2020). Tannic Acid Radicals in the Presence of Alkali Metal Salts and Their Impact on the Formation of Silicate-Phenolic Networks. ACS Applied Materials & Interfaces. ISSN 1944-8244. 12(47), s. 52457–52466. doi: 10.1021/acsami.0c16946. Fulltekst i vitenarkiv
• Chahal, Aman S; Schweikle, Manuel; Lian, Aina-Mari; Reseland, Janne Elin; Haugen, Håvard Jostein & Tiainen, Hanna (2020). Osteogenic potential of poly(ethylene glycol)-amorphous calcium phosphate composites on human mesenchymal stem cells. Journal of Tissue Engineering. ISSN 2041-7314. 11. doi: 10.1177/2041731420926840. Fulltekst i vitenarkiv
• Rumian, Lucja; Wolf-Brandstetter, Cornelia; Rößler, Sina; Reczyńska, Katarzyna; Tiainen, Hanna & Haugen, Håvard Jostein [Vis alle 8 forfattere av denne artikkelen] (2020). Sodium alendronate loaded poly(L-lactide-co-glycolide) microparticles immobilized on ceramic scaffolds for local treatment of bone defects. Regenerative Biomaterials. ISSN 2056-3426. s. 1–10. doi: 10.1093/rb/rbaa012. Fulltekst i vitenarkiv Vis sammendrag

  Bone tissue regeneration in critical-size defects is possible after implantation of a three-dimensional scaffold and can be additionally enhanced once the scaffold is enriched with drugs or other factors supporting bone remodelling and healing. Sodium alendronate (Aln), a widely used anti-osteoporosis drug, exhibits strong inhibitory effect on bone resorption performed by osteoclasts. Thus we propose a new approach for the treatment of bone defects in craniofacial region combining biocompatible titanium dioxide (TiO2) scaffolds and poly(L-lactide-co-glycolide) (PLGA) microparticles (MPs) loaded with Aln. The MPs were effectively immobilized on the surface of the scaffolds’ pore walls by human recombinant collagen. Drug release from the scaffolds was characterised by initial burst (24 ± 6% of the drug released within first 24 h) followed by a sustained release phase (on average 5 µg of Aln released per day from day 3 to day 18). In vitro tests showed that Aln in concentrations of 5 µg/mL and 2.5 µg/mL was not cytotoxic for MG-63 osteoblast-like cells (viability between 81 ± 6% to 98 ± 3% of control) but it prevented RANKL-induced formation of osteoclast-like cells from macrophages derived from peripheral blood mononuclear cells (PBMCs), as shown by reduced fusion capability and decreased TRAP 5b activity (56 ± 5% reduction in comparison control after 8 days of culture). Results show that it is feasible to design the scaffolds providing required doses of Aln inhibiting osteoclastogenesis, reducing osteoclast activity, but not affecting osteoblast functions, which may be beneficial in the treatment of critical-size bone tissue defects.

• Weber, Florian; Liao, Wei-Chih; Barrantes Bautista, Alejandro; Edèn, Mattias & Tiainen, Hanna (2019). Silicate‐Phenolic Networks: Coordination Mediated Deposition of Bioinspired Tannic Acid Coatings. Chemistry - A European Journal. ISSN 0947-6539. 25(42), s. 9870–9874. doi: 10.1002/chem.201902358. Fulltekst i vitenarkiv
• Geissler, Sebastian; Gomez-Florit, Manuel; Wiedmer, David; Barrantes Bautista, Alejandro; Petersen, Fernanda Cristina & Tiainen, Hanna (2019). In vitro performance of bioinspired phenolic nanocoatings for endosseous implant applications. ACS Biomaterials Science and Engineering. ISSN 2373-9878. 5(7), s. 3340–3351. doi: 10.1021/acsbiomaterials.9b00566. Fulltekst i vitenarkiv
• Schweikle, Manuel; Bjørnøy, Sindre Hove; Van Helvoort, Antonius; Haugen, Håvard Jostein; Sikorski, Pawel & Tiainen, Hanna (2019). Stabilisation of amorphous calcium phosphate in polyethylene glycol hydrogels. Acta Biomaterialia. ISSN 1742-7061. 90, s. 132–145. doi: 10.1016/j.actbio.2019.03.044. Fulltekst i vitenarkiv Vis sammendrag

  Acellular polymer-calcium phosphate composites are promising bone graft materials. Hydrogels are suitable for providing a temporary matrix, while calcium phosphate minerals serve as ion depots for calcium and phosphate required for de novo bone formation. Crystalline calcium phosphates are stable under biological conditions and are commonly used in such scaffolds. However, the low solubility of these phases reduces the availability of free ions and potentially obstructs the remodelling necessary for the formation of mineralised tissue. Here, we investigate two different strategies to stabilise amorphous calcium phosphates in a synthetic polyethylene glycol-based hydrogel matrix. In vitro experiments mimicking an injectable application showed that amorphous calcium phosphate (ACP) of variable stability was formed in the hydrogel matrices. In additive-free composites, ACP transformed into brushite within minutes. Citrate or zinc additives were found to stabilise the formed ACP phase to different degrees. In the presence of citrate, ACP was stable for at least 2 h before it transformed into hydroxyapatite within 3–20 days. Partial calcium substitution with zinc (Zn/Ca = 10%) produced zinc-doped ACP of high stability that did not show signs of crystallisation for at least 20 days. The presented methods and findings open new possibilities for the design of novel injectable synthetic bone graft materials. The possibility to produce ACP with tailorable stability promises great potential for creating temporary scaffolds with good osteogenic properties. Statement of significance Synthetic hydrogel-calcium phosphate (CaP) composites are promising biomaterials to replace human- and animal-derived bone scaffolds. Most reported hydrogel-CaP composite materials employ crystalline CaP phases that lack the osteoinductive properties of autograft. Stabilising amorphous calcium phosphates (ACP) could overcome this limitation, readily delivering calcium and phosphate ions and facilitating remodelling into new bone tissue. The design of synthetic hydrogel-ACP scaffolds, however, requires more understanding of the mineralisation processes in such matrices. This study presents a model system to characterise the complex mineral formation and transformation processes within a hydrogel matrix. We demonstrate a facile route to produce self-mineralising injectable synthetic hydrogels and prove two different strategies to stabilise ACP for different periods within the formed composites.

• Weber, Florian; Barrantes Bautista, Alejandro & Tiainen, Hanna (2019). Silicic acid mediated formation of tannic acid nanocoatings. Langmuir. ISSN 0743-7463. 35, s. 3327–3336. doi: 10.1021/acs.langmuir.8b04208. Fulltekst i vitenarkiv
• Klemm, Anne; Gomez-Florit, Manuel; Almeida Carvalho, Patricia; Wachendörfer, Mattis; Gomes, Manuela E. & Haugen, Håvard Jostein [Vis alle 7 forfattere av denne artikkelen] (2019). Grain boundary corrosion in TiO2 bone scaffolds doped with group II cations. Journal of the European Ceramic Society. ISSN 0955-2219. 39, s. 1577–1585. doi: 10.1016/j.jeurceramsoc.2018.12.055. Fulltekst i vitenarkiv
• Zhang, Xianbin; Tiainen, Hanna & Haugen, Håvard Jostein (2019). Comparison of titanium dioxide scaffold with commercial bone graft materials through micro-finite element modelling in flow perfusion. Medical and Biological Engineering and Computing. ISSN 0140-0118. 57(1), s. 311–324. doi: 10.1007/s11517-018-1884-2. Fulltekst i vitenarkiv Vis sammendrag

  TiO2 scaffolds have previously shown to have promising osteoconductive properties in previous in vivo experiments. Appropriate mechanical stimuli can further promote this osteoconductive behaviour. However, the complex mechanical environment and the mechanical stimuli enhancing bone regeneration for porous bioceramics have not yet been fully elucidated. This paper aims to compare and evaluate mechanical environment of TiO2 scaffold with three commercial CaP biomaterials, i.e. Bio-Oss, Cerabone and Maxresorb under simulated perfusion culture conditions. The solid phase and fluid phase were modelled as linear elastic material and Newtonian fluid, respectively. The mechanical stimulus was analysed within these porous scaffolds quantitatively. The results showed that the TiO2 had nearly heterogeneous stress distributions, however lower effective Young’s modulus than Cerabone and Maxresorb. The permeability and wall shear stress (WSS) for the TiO2 scaffold was significantly higher than other commercial bone substitute materials. Maxresorb and Bio-Oss showed lowest permeability and local areas of very high WSS. The detailed description of the mechanical performance of these scaffolds could help researchers to predict cell behaviour and to select the most appropriate scaffold for different in vitro and in vivo performances.

• Bossard, Cedric; Granel, Henri; Jallot, Édouard; Vial, Christophe; Tiainen, Hanna & Wittrant, Yohann [Vis alle 7 forfattere av denne artikkelen] (2018). Polycaprolactone / bioactive glass hybrid scaffolds for bone regeneration. Biomedical Glasses. ISSN 2299-3932. 4, s. 108–122. doi: 10.1515/bglass-2018-0010. Fulltekst i vitenarkiv
• Wiedmer, David; Cui, Chen; Weber, Florian; Petersen, Fernanda Cristina & Tiainen, Hanna (2018). Antibacterial Surface Coating for Bone Scaffolds Based on the Dark Catalytic Effect of Titanium Dioxide. ACS Applied Materials & Interfaces. ISSN 1944-8244. 10(42), s. 35784–35793. doi: 10.1021/acsami.8b12623. Fulltekst i vitenarkiv
• Haugen, Sigrid; He, Jianying; Sundaresan, Alamelu; Stunes, Astrid Kamilla; Aasarød, Kristin Matre & Tiainen, Hanna [Vis alle 9 forfattere av denne artikkelen] (2018). Adiponectin reduces bone stiffness; verified in a 3D artificial human bone model in vitro. Frontiers in Endocrinology. ISSN 1664-2392. 9:236, s. 1–12. doi: 10.3389/fendo.2018.00236. Fulltekst i vitenarkiv
• Chahal, Aman Singh; Schweikle, Manuel; Heyward, Catherine Anne & Tiainen, Hanna (2018). Attachment and spatial organisation of human mesenchymal stem cells on poly(ethylene glycol) hydrogels. Journal of The Mechanical Behavior of Biomedical Materials. ISSN 1751-6161. 84, s. 46–53. doi: 10.1016/j.jmbbm.2018.04.025. Fulltekst i vitenarkiv
• Schweikle, Manuel; Zinn, Thomas; Lund, Reidar & Tiainen, Hanna (2018). Injectable synthetic hydrogel for bone regeneration: Physicochemical characterisation of a high and a low pH gelling system. Materials Science and Engineering C: Materials for Biological Applications. ISSN 0928-4931. 90, s. 67–76. doi: 10.1016/j.msec.2018.04.049. Fulltekst i vitenarkiv
• Verket, Anders; Lyngstadaas, Ståle Petter; Tiainen, Hanna; Rønold, Hans Jacob & Wohlfahrt, Johan Caspar (2018). Impact of particulate deproteinized bovine bone mineral and porous titanium granules on early stability and osseointegration of dental implants in narrow marginal circumferential bone defects. International Journal of Oral & Maxillofacial Surgery. ISSN 0901-5027. 47(8), s. 1086–1094. doi: 10.1016/j.ijom.2018.02.007. Fulltekst i vitenarkiv
• Klemm, Anne & Tiainen, Hanna (2018). Coagulated concentrated anatase slurry leads to improved strength of ceramic TiO⁠2 bone scaffolds. Ceramics International. ISSN 0272-8842. 44(6), s. 6265–6271. doi: 10.1016/j.ceramint.2018.01.013. Fulltekst i vitenarkiv
• Wiedmer, David; Petersen, Fernanda Cristina; Lönn-Stensrud, Jessica & Tiainen, Hanna (2017). Antibacterial effect of hydrogen peroxide - titanium dioxide suspensions in the decontamination of rough titanium surfaces. Biofouling (Print). ISSN 0892-7014. 33(6), s. 451–459. doi: 10.1080/08927014.2017.1322585. Fulltekst i vitenarkiv
• Rumian, Lucja; Tiainen, Hanna; Cibor, Urszula; Krok-Borkowicz, Małgorzata; Brzychczy-Włoch, Monika & Haugen, Håvard Jostein [Vis alle 7 forfattere av denne artikkelen] (2017). Ceramic scaffolds with immobilized vancomycin-loaded poly(lactide-co-glycolide) microparticles for bone defects treatment. Materials Letters. ISSN 0167-577X. 190, s. 67–70. doi: 10.1016/j.matlet.2016.12.113.
• Rumian, Lucja; Tiainen, Hanna; Cibor, Urszula; Krok, Malgorzata; Brzychczy-Wloch, Monika & Haugen, Håvard Jostein [Vis alle 7 forfattere av denne artikkelen] (2016). Ceramic scaffolds enriched with gentamicin loaded poly(lactide-co-glycolide) microparticles for prevention and treatment of bone tissue infections. Materials Science and Engineering C: Materials for Biological Applications. ISSN 0928-4931. doi: 10.1016/j.msec.2016.07.065.
• Geissler, Sebastian; Barrantes, Alejandro; Tengvall, Pentti; Messersmith, Phillip B. & Tiainen, Hanna (2016). Deposition kinetics of bioinspired phenolic coatings on titanium surfaces . Langmuir. ISSN 0743-7463. 32, s. 8050–8060. doi: 10.1021/acs.langmuir.6b01959. Fulltekst i vitenarkiv
• Wiedmer, David; Sagstuen, Einar; Welch, Ken; Haugen, Håvard Jostein & Tiainen, Hanna (2016). Oxidative power of aqueous non-irradiated TiO2-H2O2 suspensions: Methylene blue degradation and the role of reactive oxygen species. Applied Catalysis B: Environmental. ISSN 0926-3373. 198, s. 9–15. doi: 10.1016/j.apcatb.2016.05.036. Vis sammendrag

  In the present study, the degradation of methylene blue in non-irradiated TiO2-H2O2 suspensions was investigated. Five commercially available catalysts were characterised (BET surface area, zeta potential, hydrodynamic diameter) and their oxidative power was assessed by means of the degradation of methylene blue. A subsequent EPR study was made to verify and identify potential oxidative radicals. The results showed that all suspensions could degrade methylene blue significantly stronger compared to hydrogen peroxide alone. A broad variation between the different catalysts in their capability to adsorb dye molecules was found which was essential for decomposition of methylene blue in darkness. The highest degradation rate of all samples was found for Degussa P25 at neutral pH. EPR studies of this sample verified the presence of oxygen centred radicals namely hydroxyl (radical dotOH) and superoxide radicals (O2−radical dot/radical dotOOH). Non-irradiated TiO2-H2O2 systems show great potential not only in dye removal applications but also in the field of disinfection where low concentrations of hydrogen peroxide are required and irradiation may not be feasible.

• Geissler, Sebastian; Tiainen, Hanna & Haugen, Håvard Jostein (2016). Effect of cathodic polarization on coating doxycycline on titanium surfaces. Materials Science and Engineering C: Materials for Biological Applications. ISSN 0928-4931. 63, s. 359–366. doi: 10.1016/j.msec.2016.03.012.
• Müller, Benjamin; Haugen, Håvard Jostein; Nilsen, Ola & Tiainen, Hanna (2016). Atomic layer deposited TiO2 protects porous ceramic foams from grain boundary corrosion. Corrosion Science. ISSN 0010-938X. 106, s. 35–42. doi: 10.1016/j.corsci.2016.01.023. Vis sammendrag

  Intergranular corrosion causes a significant loss of compressive strength in highly porous TiO2 foams. In this study, the resistance of conformal atomic layer deposited (ALD) coatings against intergranular corrosion has been investigated. ALD coated TiO2 foams maintained 107% ± 20% and 94% ± 10% of their initial compressive strength for films deposited at 150 °C and 250 °C, respectively. After an immersion period of 28 days in 1 mM HCl, amorphous films showed greater potential in corrosion protection of polycrystalline TiO2 foams. However, anatase coatings with sufficient protection may be more beneficial for cell-material interactions.

• Crica, Livia Elena; Wengenroth, Jonas; Tiainen, Hanna; Ionita, Mariana & Haugen, Håvard Jostein (2016). Enhanced X-ray absorption for micro-CT analysis of low density polymers. Journal of Biomaterials Science. Polymer Edition. ISSN 0920-5063. 27(9), s. 805–823. doi: 10.1080/09205063.2016.1152856. Vis sammendrag

  X-ray microtomography (micro-CT), one of the most resourceful instruments for high resolution 3D analysis, can provide qualitative and quantitative accurate structural and compositional information for a broad range of materials. Yet its contribution to the field of biopolymeric materials science is often limited by low imaging contrast due to scarce X-ray attenuation features, particularly for sponges and foam-like structures. This limitation can be overcome to some extent by adjusting the working parameters of micro-CT equipment. However, such approach also facilitates noise and artefacts, and solving the signal-to-noise trade-off has been always problematic. Searching for alternatives turns one’s attention towards the improvement of X-ray attenuation features. While several studies report the use of contrast agents for biological materials, studies to integrate multiple micro-CT approaches for biopolymers were not conducted so far. This method paper is thus aimed to serve as a platform for micro-CT analysis of low X-ray absorptive polymers. Here, several contrast enhancing artifices were developed and trialled on gelatin and poly(vinyl alcohol) biopolymer composites (GP). Accordingly, GP were modified with iodine, barium, silver-based chemicals and hexa(methyl disilazane) by two different methods, i.e. addition of high atomic number chemicals during materials synthesis and post-synthesis staining, respectively. Consequently, cross-sectional scanning electron microscopy was emerged as complementary characterization, aimed to confirm the reproducibility of samples morphological features. The most versatile methods were barium chloride additive incorporation and iodine staining coupled with hexa(methyl disilazane) chemical drying. Both methods significantly improved the X-ray absorbance of our polymeric samples, providing better contrast of micro-CT tomograms.

• Verket, Anders; Müller, Benjamin; Wohlfahrt, Johan Caspar; Lyngstadaas, Ståle Petter; Ellingsen, Jan Eirik & Haugen, Håvard Jostein [Vis alle 7 forfattere av denne artikkelen] (2016). TiO2 scaffolds in peri-implant dehiscence defects: an experimental pilot study. Clinical Oral Implants Research. ISSN 0905-7161. 27(10), s. 1200–1206. doi: 10.1111/clr.12725.
• Müller, Benjamin; Haugen, Håvard Jostein; Simonsen, Siri Lene & Tiainen, Hanna (2016). Grain boundary corrosion of highly porous ceramic TiO<sub>2</sub> foams is reduced by annealing and quenching. Journal of the European Ceramic Society. ISSN 0955-2219. 36(1), s. 179–188. doi: 10.1016/j.jeurceramsoc.2015.09.014.
• Ionita, Mariana; Crica, Livia E; Tiainen, Hanna; Haugen, Håvard Jostein; Vasile, Eugenia & Dinescu, Sorina [Vis alle 8 forfattere av denne artikkelen] (2015). Gelatin-poly(vinyl alcohol) porous biocomposites reinforced with graphene oxide as biomaterials. Journal of materials chemistry. B. ISSN 2050-750X. 4(2), s. 282–291. doi: 10.1039/c5tb02132d.
• Xing, Rui; Witsø, Ingun Lund; Jugowiec, David; Tiainen, Hanna; Shabestari, Maziar & Lyngstadaas, Ståle Petter [Vis alle 8 forfattere av denne artikkelen] (2015). Antibacterial effect of doxycycline-coated dental abutment surfaces. Biomedical Materials. ISSN 1748-6041. 10(5), s. 55003–55012. doi: 10.1088/1748-6041/10/5/055003. Vis sammendrag

  Biofilm formation on dental abutment may lead to peri-implant mucositis and subsequent peri-implantitis. These cases are clinically treated with antibiotics such as doxycycline (Doxy). Here we used an electrochemical method of cathodic polarization to coat Doxy onto the outer surface of a dental abutment material. The Doxy-coated surface showed a burst release in phosphate-buffered saline during the first 24 h. However, a significant amount of Doxy remained on the surface for at least 2 weeks especially on a 5 mA–3 h sample with a higher Doxy amount, suggesting both an initial and a long-term bacteriostatic potential of the coated surface. Surface chemistry was analyzed by x-ray photoelectron spectroscopy and secondary ion mass spectrometry. Surface topography was evaluated by field emission scanning electron microscopy and blue-light profilometry. Longer polarization time from 1 h to 5 h and higher current density from 1 to 15 mA cm−2 resulted in a higher amount of Doxy on the surface. The surface was covered by a layer of Doxy less than 100 nm without significant changes in surface topography. The antibacterial property of the Doxy-coated surface was analyzed by biofilm and planktonic growth assays using Staphylococcus epidermidis. Doxy-coated samples reduced both biofilm accumulation and planktonic growth in broth culture, and also inhibited bacterial growth on agar plates. The antibacterial effect was stronger for samples of 5 mA–3 h coated with a higher amount of Doxy compared to that of 1 mA–1 h. Accordingly, an abutment surface coated with Doxy has potential for preventing bacterial colonization when exposed to the oral cavity. Doxy-coating could be a viable way to control peri-implant mucositis and prevent its progression into peri-implantitis.

• Pullisaar, Helen; Verket, Anders; Szoke, Krisztina; Tiainen, Hanna; Haugen, Håvard Jostein & Brinchmann, Jan E. [Vis alle 8 forfattere av denne artikkelen] (2015). Alginate hydrogel enriched with enamel matrix derivative to target osteogenic cell differentiation in TiO2 scaffolds. Journal of Tissue Engineering. ISSN 2041-7314. 6. doi: 10.1177/2041731415575870. Fulltekst i vitenarkiv
• Bohner, Marc; Tiainen, Hanna; Michel, Pascal & Döbelin, Nicola (2015). Design of an inorganic dual-paste apatite cement using cation exchange. Journal of materials science. Materials in medicine. ISSN 0957-4530. 26(2), s. 1–13. doi: 10.1007/s10856-015-5400-z.
• Müller, Benjamin; Reseland, Janne Elin; Haugen, Håvard Jostein & Tiainen, Hanna (2015). Cell growth on pore-graded biomimetic TiO2 bone scaffolds. Journal of Biomaterials Applications. ISSN 0885-3282. 29(9), s. 1284–1295. doi: 10.1177/0885328214559859.
• Rumian, Lucja; Reczynska, Katarzyna; Wrona, Małgorzata; Tiainen, Hanna; Haugen, Håvard Jostein & Pamula, Elzbieta (2015). The influence of sintering conditions on microstructure and mechanical properties of titanium dioxide scaffolds for the treatment of bone tissue defects. Acta of Bioengineering and Biomechanics. ISSN 1509-409X. 17(1), s. 3–9. doi: 10.5277/ABB-00129-2014-02. Vis sammendrag

  In this study the attempts to improve mechanical properties of highly-porous titanium dioxide scaffolds produced by polymer sponge replication method were investigated. Particularly the effect of two-step sintering at different conditions on microstructure (studied by microcomputed tomography and scanning electron microscopy) and mechanical properties (compression test) of the scaffolds were analysed. Our experiments showed that the most appropriate conditions of manufacturing were when the scaffolds were heat-treated at 1500 oC for 1 h followed by sintering at 1200 oC for 20 h. Such scaffolds exhibited the highest compressive strength which was correlated with the highest linear density and the lowest size of grains. Moreover, grain size distribution was narrower with predominating fraction of fine grains 10-20 µm in size. Smaller grains and higher linear density suggested that in this case densification process prevailed undesirable process of grain coarsening, what finally resulted in improved mechanical properties of the scaffolds.

Se alle arbeider i Cristin

• Tiainen, Hanna; Galli, Enzo; Dumay, Yann & Barrantes Bautista, Alejandro (2024). Desorption of salivary pellicle and primer adsorption on zirconium dioxide dental ceramics.
• Tiainen, Hanna (2023). Biofunctionalized cellulose membrane for guided tissue regeneration.
• Oreja Remartínez, Enrique; Tiainen, Hanna; Rogala, Agnieszka; Barrantes Bautista, Alejandro & Zaytseva-Zotova, Daria (2023). Tannic Acid/Polyamino acid Layer-by-Layer coating and their interaction with human cells.
• Rogala, Agnieszka; Zaytseva-Zotova, Daria; Barrantes Bautista, Alejandro & Tiainen, Hanna (2023). QCM-D combined with live cell imaging reveals inverse correlation between cell adhesion to tannic acid nanocoatings and serum concentration in medium .
• Zaytseva-Zotova, Daria; Singh, Simon; Haideri, Frozan; Elkafas, Nadin Abdelwahab Mohamed Abdou; Barrantes Bautista, Alejandro & Singh, Preet Bano [Vis alle 7 forfattere av denne artikkelen] (2023). Capsaicin-loaded microdroplets for the treatment of burning mouth syndrome.
• Weber, Florian; Barrantes Bautista, Alejandro & Tiainen, Hanna (2021). Antioxidant polyphenolic implant coatings for improved wound healing.
• Weber, Florian; Barrantes Bautista, Alejandro & Tiainen, Hanna (2021). Can plant polyphenol inspired surface modifications improve tissue integration of titanium implants?
• Weber, Florian; Sagstuen, Einar & Tiainen, Hanna (2021). Influence of monovalent ions on the coating formation of tannic acid.
• Weber, Florian; Sagstuen, Einar & Tiainen, Hanna (2020). Chemistry of Silica-Phenolic Networks.
• Weber, Florian; Barrantes Bautista, Alejandro & Tiainen, Hanna (2019). Plant polyphenolic inspired anti-inflammatory and anti-bacterial surface modifications for dental implants.
• Weber, Florian; Liao, Wei-Chih; Barrantes Bautista, Alejandro; Edèn, Mattias & Tiainen, Hanna (2019). Silicic acid mediated formation of tannic acid coatings.
• Klemm, Anne; Haugen, Håvard Jostein & Tiainen, Hanna (2018). Grain boundary corrosion in Sr and Ca doped TiO2 bone scaffold. Vis sammendrag

  Porous TiO2 scaffolds as a bone support structure showed high porosity combined with high compressive strength1. Doping ceramic scaffolds with Ca or Sr leads to even higher compressive strength and formation of impurity rich grain boundaries2. Grain boundary dissolution can occur during bone healing, because of a local pH drop3. To improve the corrosion resistance of cationic doped TiO2 scaffolds, the objective of this study was to investigate the corrosion depending on doping concentration to evaluate corrosion mechanisms.

• Schweikle, Manuel; Bjørnøy, Sindre Hove; Lyngstadaas, Ståle Petter; Haugen, Håvard Jostein; Van Helvoort, Antonius & Sikorski, Pawel [Vis alle 7 forfattere av denne artikkelen] (2018). Self-mineralising synthetic injectable hydrogels for bone repair.
• Tiainen, Hanna; Wiedmer, David & Petersen, Fernanda Cristina (2018). Biofilm formation and planktonic growth of S. epidermidis in the presence of phenolic compounds tannic acid and pyrogallol.
• Wiedmer, David; Cui, Chen; Petersen, Fernanda Cristina & Tiainen, Hanna (2018). Antibacterial sol-gel coating for bone scaffolds based on the dark catalytic effect of titanium dioxide.
• Weber, Florian; Tiainen, Hanna & Barrantes Bautista, Alejandro (2018). pH dependent coating formation of tannic acid on titanium surfaces.
• Schweikle, Manuel; Bjørnøy, Sindre Hove; Zinn, Thomas; Lund, Reidar; Sikorski, Pawel & Tiainen, Hanna (2017). In situ mineralising injectable hydrogels for bone repair.
• Schweikle, Manuel; Zinn, Thomas; Lund, Reidar & Tiainen, Hanna (2017). Injectable hydrogel scaffold for regenerative treatment of peri-implantitis defects.
• Schweikle, Manuel; Bjørnøy, Sindre Hove; Zinn, Thomas; Lund, Reidar; Sikorski, Pawel & Tiainen, Hanna (2017). Mineralised injectable hydrogels for bone regeneration.
• Weber, Florian; Tiainen, Hanna & Barrantes Bautista, Alejandro (2017). pH dependent coating formation of tannic acid on titanium surfaces.
• Bjørnøy, Sindre Hove; Bassett, David; Schweikle, Manuel; Strand, Berit Løkensgard; Tiainen, Hanna & Ucar, Seniz [Vis alle 8 forfattere av denne artikkelen] (2017). Bioinspired mineralization of hydrogels.
• Gomez-Florit, Manuel; Geissler, Sebastian; Wiedmer, David; Petersen, Fernanda Cristina & Tiainen, Hanna (2017). Multifunctional polyphenol nanocoatings for improved tissue integration of dental implants.
• Geissler, Sebastian; Gomez-Florit, Manuel; Barrantes, Alejandro & Tiainen, Hanna (2016). Deposition kinetics of bioinspired polyphenol nanocoatings and their effect on osteogenic differentiation on titanium surfaces.
• Wiedmer, David; Tiainen, Hanna; Petersen, Fernanda Cristina & Haugen, Håvard Jostein (2016). H2O2-TiO2 as potential treatment for peri-implantitis – an in vitro study.
• Wiedmer, David; Tiainen, Hanna; Lönn-Stensrud, Jessica; Petersen, Fernanda Cristina & Haugen, Håvard Jostein (2016). TiO2-H2O2 as decontamination agent - an in vitro study.
• Schweikle, Manuel; Hartvig, Rune Andersen; Zinn, Thomas & Tiainen, Hanna (2016). Tuning gelation kinetics and mechanical properties of a synthetic extracellular matrix substitute.
• Schweikle, Manuel; Hartvig, Rune; Zinn, Thomas & Tiainen, Hanna (2016). Injectable hydrogel scaffold for bone regeneration: gelation and mechanical properties.
• Geissler, Sebastian; Barrantes, Alejandro; Tengvall, Pentti & Tiainen, Hanna (2016). Coating kinetics of bioinspired phenolic films on titanium surfaces.
• Geissler, Sebastian; Barrantes, Alejandro; Tengvall, Pentti & Tiainen, Hanna (2016). Reaction kinetics of bioinspired phenolic coatings on titanium surfaces.
• Tiainen, Hanna; Müller, Benjamin; Campos, Lluis; Ramis, Joana Maria; Nilsen, Ola & Haugen, Håvard Jostein (2016). Atomic layer deposited titanium dioxide layer protects highly porous ceramic foams from grain boundary corrosion.
• Tiainen, Hanna; Müller, Benjamin; Campos, Lluis; Ramis, Joana Maria; Nilsen, Ola & Haugen, Håvard Jostein (2016). Atomic layer deposited TiO2 protects highly porous ceramic bone scaffolds from grain boundary corrosion.
• Klemm, Anne & Tiainen, Hanna (2016). Investigation of ionic strength on aqueous TiO2 slurries and microstructure of sintered TiO2 bone scaffolds.
• Wiig, Elisabeth; Tiainen, Hanna; Lian, Aina-Mari; Mann, Vivek; Sundaresan, Alamelu & Haugen, Håvard Jostein [Vis alle 8 forfattere av denne artikkelen] (2015). Effect of microgravity on bone cells in bioinspired ceramic TiO2 scaffolds.
• Chahal, Aman Singh; Schweikle, Manuel; Tiainen, Hanna; Koldsland, Odd Carsten; Reseland, Janne Elin & Haugen, Håvard Jostein (2015). Cell-responsive Biomimetic Extracellular Matrix Material for Bone and Dental Pulp Tissue Regeneration.
• Schweikle, Manuel; Tiainen, Hanna & Haugen, Håvard Jostein (2015). Poly(lactic-co-glycolic acid)-based nanoparticles as drug delivery systems for doxycycline hyclate and simvastatin for the treatment of inflammation-caused bone resorption.
• Geissler, Sebastian; Tiainen, Hanna & Haugen, Håvard Jostein (2015). Electrochemical immobilization of doxycycline on titanium surfaces.
• Wiedmer, David; Recklies, Sandra; Bossard, Cedric; Tiainen, Hanna & Haugen, Håvard Jostein (2015). Antibacterial sol-gel coating for ceramic TiO2 scaffolds for bone regeneration.
• Tiainen, Hanna; Geissler, Sebastian; Lund, Reidar & Haugen, Håvard Jostein (2015). pH triggered aggregation of a structurally disordered polyproline peptide.
• Rumian, Lucja; Tiainen, Hanna; Posadowska, Urszula; Brzychczy-Włoch, Monika; Haugen, Håvard Jostein & Pamula, Elzbieta (2015). Ceramic scaffolds containing gentamicin sulphate loaded poly(lactic-co-glycolic acid) microparticles for bone tissue engineering.
• Wiedmer, David; Tiainen, Hanna; Sagstuen, Einar & Haugen, Håvard Jostein (2015). TiO2 nanoparticles as antibacterial debridement strategy for permanent infected dental implants.
• Tiainen, Hanna; Pieprzak, Kaja & Reczyńska, Katarzyna (2015). Effect of sintering conditions on structural and biological properties of TiO2 bone scaffolds.
• Longela, Simon Makusudi; Haugen, Håvard Jostein; Tiainen, Hanna; Lyngstadaas, Ståle Petter & Reseland, Janne Elin (2015). Biomolecular coating of nickel-titanium memory shape metals. European Cells and Materials. ISSN 1473-2262. 29.
• Longela, Simon Makusudi; Haugen, Håvard Jostein & Tiainen, Hanna (2015). Biomolecular coating of nickel-titanium memory shape metals.
• Longela, Simon Makusudi; Haugen, Håvard Jostein; Hartvig, Rune & Tiainen, Hanna (2015). Biomolecular coating of Nickel-titanium Shape memory metals.
• Longela, Simon Makusudi; Haugen, Håvard Jostein; Tiainen, Hanna & Hartvig, Rune (2015). Surface treatment of pseudoelastic nickel-titanium.
• Wiedmer, David Julian; Tiainen, Hanna & Haugen, Håvard Jostein (2015). Antibacterial debridement strategy for infected permanent dental implants.
• Tiainen, Hanna; Geissler, Sebastian; Lund, Reidar & Haugen, Håvard Jostein (2015). pH triggered self-assembly and aggregation of a structurally disordered proline-rich polypeptide.
• Schweikle, Manuel; Tiainen, Hanna & Haugen, Håvard Jostein (2015). Development of poly(lactic-co-glycolic acid)-based nanoparticles as a drug delivery system for bone regeneration implants.
• Geissler, Sebastian; Tiainen, Hanna & Haugen, Håvard Jostein (2015). Electrochemical attachment of doxycycline to titanium surfaces.
• Geissler, Sebastian; Tiainen, Hanna; Lund, Reidar & Haugen, Håvard Jostein (2015). pH triggered aggregation of an intrinsically disordered proline-rich peptide.
• Tiainen, Hanna (2014). Ceramic TiO2 scaffolds for bone regeneration.
• Tiainen, Hanna (2014). Biomimetic materials for fixing bone and teeth - lessons learned from nature.
• Tiainen, Hanna; Reczyńska, Katarzyna & Haugen, Håvard Jostein (2014). Influence of sintering conditions on the properties of TiO2 bone scaffolds.
• Pullisaar, Helen; Verket, Anders; Szoke, Krisztina; Tiainen, Hanna; Haugen, Håvard Jostein & Brinchmann, Jan E. [Vis alle 8 forfattere av denne artikkelen] (2014). Extracellular matrix coated ceramic scaffold enhance osteoblast differentiation.

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