Helen Pullisaar

• Pullisaar, Helen; Cattaneo, Paolo M.; Gera, Arwa; Sankiewicz, Monika; Bilińska, Małgorzata & Vandevska-Radunovic, Vaska [Vis alle 7 forfattere av denne artikkelen] (2024). Stability, survival, patient satisfaction, and cost-minimization of CAD/CAM versus conventional multistranded fixed retainers in orthodontic patients: a 2-year follow-up of a two-centre randomized controlled trial. European Journal of Orthodontics. ISSN 0141-5387. 46(2). doi: 10.1093/ejo/cjae006.
• Namura, Yasuhiro; Pullisaar, Helen; Holm, Heidi Vanessa; Syverud, Morten; Mulic, Aida Gacic & Vandevska-Radunovic, Vaska (2023). Elemental composition, corrosion resistance and mechanical properties of computer-aided design and computer-aided manufacturing fixed retainers versus conventional fixed retainers. Journal of Oral Science. ISSN 1343-4934. 66(2), s. 107–110. doi: 10.2334/josnusd.23-0303.
• Yang, Yang; Pullisaar, Helen; Stunes, Astrid Kamilla; Parreiras Nogueira, Liebert; Syversen, Unni & Reseland, Janne Elin (2023). Irisin reduces orthodontic tooth movement in rats by promoting the osteogenic potential in the periodontal ligament. European Journal of Orthodontics. ISSN 0141-5387. 45(6), s. 842–853. doi: 10.1093/ejo/cjad021. Fulltekst i vitenarkiv
• Yaddehi Gamage, Thilini Hansamali Gamage; Grabmayr, Herwig; Horvath, Ferdinand; Fahrner, Marc; Misceo, Doriana & Louch, William Edward [Vis alle 21 forfattere av denne artikkelen] (2023). A single amino acid deletion in the ER Ca2+ sensor STIM1 reverses the in vitro and in vivo effects of the Stormorken syndrome-causing R304W mutation. Science Signaling. ISSN 1945-0877. 16(771). doi: 10.1126/scisignal.add0509. Fulltekst i vitenarkiv
• Yang, Yang; Geng, Tianxiang; Samara, Athina; Olstad, Ole Kristoffer; He, Jianying & Agger, Anne Eriksson [Vis alle 11 forfattere av denne artikkelen] (2023). Recombinant irisin enhances the extracellular matrix formation, remodeling potential, and differentiation of human periodontal ligament cells cultured in 3D. Journal of Periodontal Research. ISSN 0022-3484. 58(2), s. 336–349. doi: 10.1111/jre.13094. Vis sammendrag

  Abstract Background Irisin is expressed in human periodontal ligament (hPDL), and its administration enhances growth, migration and matrix deposition in hPDL cells cultured in monolayers in vitro. Objectives To identify whether irisin affects the gene expression patterns directing the morphology, mechanical properties, extracellular matrix (ECM) formation, osteogenic activity and angiogenic potential in hPDL cell spheroids cultured in 3D. Materials and Methods Spheroids of primary human hPDL cells were generated in a rotational 3D culture system and treated with or without irisin. The gene expression patterns were evaluated by Affymetrix microarrays. The morphology of the spheroids was characterized using histological staining. Mechanical properties were quantified by nanoindentation. The osteogenic and angiogenic potential of spheroids were assessed through immunofluorescence staining for collagen type I, periostin fibronectin and von Willebrand factor (vWF), and mRNA expression of osteogenic markers. The secretion of multiple myokines was evaluated using Luminex immunoassays. Results Approximately 1000 genes were differentially expressed between control and irisin-treated groups by Affymetrix. Several genes related to ECM organization were differentially expressed, and multiple deubiquitinating enzymes were upregulated in the irisin-exposed samples analyzed. These represent cellular and molecular mechanisms indicative of a role for irisin in tissue remodeling. Irisin induced a rim-like structure on the outer region of the hPDL spheroids, ECM-related protein expression and the stiffness of the spheroids were enhanced by irisin. The expression of osteogenic and angiogenetic markers was increased by irisin. Conclusions Irisin altered the morphology in primary hPDL cell-derived spheroids, enhanced its ECM deposition, mechanical properties, differentiation and remodeling potential.

• Gera, Arwa; Pullisaar, Helen; Cattaneo, Paolo M.; Gera, Shadi; Vandevska-Radunovic, Vaska & Cornelis, Marie (2022). Stability, survival, and patient satisfaction with CAD/CAM versus conventional multistranded fixed retainers in orthodontic patients: a 6-month follow-up of a two-centre randomized controlled clinical trial. European Journal of Orthodontics. ISSN 0141-5387. 45(1), s. 58–67. doi: 10.1093/ejo/cjac042. Fulltekst i vitenarkiv
• Yang, Yang; Pullisaar, Helen; Landin, Maria Augusta Dos S Silva; Heyward, Catherine Anne; Schröder, Maria & Geng, Tianxiang [Vis alle 8 forfattere av denne artikkelen] (2021). FNDC5/irisin is expressed and regulated differently in human periodontal ligament cells, dental pulp stem cells and osteoblasts. Archives of Oral Biology. ISSN 0003-9969. doi: 10.1016/j.archoralbio.2021.105061. Fulltekst i vitenarkiv
• Yaddehi Gamage, Thilini Hansamali Gamage; Lengle, Emma; Gunnes, Gjermund; Pullisaar, Helen; Holmgren, Asbjørn & Reseland, Janne Elin [Vis alle 16 forfattere av denne artikkelen] (2019). STIM1 R304W in mice causes subgingival hair growth and an increased fraction of trabecular bone. Cell Calcium. ISSN 0143-4160. 85. doi: 10.1016/j.ceca.2019.102110.
• Pullisaar, Helen; Colaianni, Graziana; Lian, Aina-Mari; Vandevska-Radunovic, Vaska; Grano, Maria & Reseland, Janne Elin (2019). Irisin promotes growth, migration and matrix formation in human periodontal ligament cells. Archives of Oral Biology. ISSN 0003-9969. doi: 10.1016/j.archoralbio.2019.104635. Fulltekst i vitenarkiv
• Khanifam, Pejman; Pullisaar, Helen & Risheim, Helge (2018). Local facial atrophy and permanent anesthesia of right upper lip following subcutaneous extrusion of chlorhexidine digluconate. Oral and Maxillofacial Surgery Cases. ISSN 2214-5419. 5(1). doi: 10.1016/j.omsc.2018.10.009. Fulltekst i vitenarkiv
• Bizelli-Silveira, Carolina; Pullisaar, Helen; Abildtrup, Lisbeth A.; Andersen, Ole Z.; Spin-Neto, Rubens & Foss, Morten [Vis alle 7 forfattere av denne artikkelen] (2018). Strontium enhances proliferation and osteogenic behavior of periodontal ligament cells in vitro. Journal of Periodontal Research. ISSN 0022-3484. doi: 10.1111/jre.12601. Fulltekst i vitenarkiv
• Pullisaar, Helen; Verket, Anders; Szoke, Krisztina; Tiainen, Hanna; Haugen, Håvard Jostein & Brinchmann, Jan E. [Vis alle 8 forfattere av denne artikkelen] (2015). Alginate hydrogel enriched with enamel matrix derivative to target osteogenic cell differentiation in TiO2 scaffolds. Journal of Tissue Engineering. ISSN 2041-7314. 6. doi: 10.1177/2041731415575870. Fulltekst i vitenarkiv
• Mosti, Mats Peder; Stunes, Astrid Kamilla; Ericsson, Madelene; Pullisaar, Helen; Reseland, Janne Elin & Shabestari, Maziar [Vis alle 8 forfattere av denne artikkelen] (2014). Effects of the peroxisome proliferator-activated receptor (PPAR) delta agonist GW501516 on bone and muscle in ovariectomized rats. Endocrinology. ISSN 0013-7227. 155(6), s. 2178–2189. doi: 10.1210/en.2013-1166.
• Pullisaar, Helen; Reseland, Janne Elin; Haugen, Håvard Jostein; Brinchmann, Jan E. & Østrup, Esben (2014). Simvastatin coating of TiO2 scaffold induces osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells. Biochemical and Biophysical Research Communications - BBRC. ISSN 0006-291X. 447(1), s. 139–144. doi: 10.1016/j.bbrc.2014.03.133.
• Rubert, Marina; Pullisaar, Helen; Gomez-Florit, Manuel; Ramis, Joana Maria; Tiainen, Hanna & Haugen, Håvard Jostein [Vis alle 8 forfattere av denne artikkelen] (2013). Effect of TiO2 scaffolds coated with alginate hydrogel containing a proline-rich peptide on osteoblast growth and differentiation in vitro. Journal of Biomedical Materials Research. Part A. ISSN 1549-3296. 101A(6), s. 1768–1777. doi: 10.1002/jbm.a.34458. Vis sammendrag

  The aim of this study was to investigate the effect of TiO2 scaffold (SC) coated with an alginate hydrogel containing a proline-rich peptide (P2) on osteoblast proliferation and differentiation in vitro. Peptide release was evaluated and a burst release was observed during the first hours of incubation, and then progressively released overtime. No changes were observed in the cytotoxicity after 48h of seeding MC3T3-E1 cells on the coated and uncoated TiO2 SC. The amount of cells after 7 days was higher on uncoated TiO2 SC than on alginate-coated TiO2 SC, measured by DNA content and SEM imaging. In addition, while lower expression of integrin beta1 was detected for alginate-coated TiO2 SC at this time point, similar gene expression was observed for other integrins, fibronectin-1, and several osteoblast differentiation markers. After 21 days, gene expression of integrin beta3, fibronectin-1, osterix and collagen-I was increased in alginate-coated compared to TiO2 SC. Moreover, increased gene expression of integrin alpha8, bone morphogenetic protein 2, interleukin-6 and collagen-I was found on P2 alginate-coated TiO2 SC compared to alginate-coated TiO2 SC. In conclusion, our results indicate that alginate-coated TiO2 SC can act as a matrix for delivery of proline-rich peptides increasing osteoblast differentiation.

• Pullisaar, Helen; Tiainen, Hanna; Landin, Maria Augusta Dos S Silva; Lyngstadaas, Ståle Petter; Haugen, Håvard Jostein & Reseland, Janne Elin [Vis alle 7 forfattere av denne artikkelen] (2013). Enhanced in vitro osteoblast differentiation on TiO2 scaffold coated with alginate hydrogel containing simvastatin. Journal of Tissue Engineering. ISSN 2041-7314. 4, s. 1–13. doi: 10.1177/2041731413515670. Fulltekst i vitenarkiv Vis sammendrag

  The aim of this study was to develop a three-dimensional porous bone graft material as vehicle for simvastatin delivery and to investigate its effect on primary human osteoblasts from three donors. Highly porous titanium dioxide (TiO2) scaffolds were submerged into simvastatin containing alginate solution. Microstructure of scaffolds, visualized by scanning electron microscopy and micro-computed tomography, revealed an evenly distributed alginate layer covering the surface of TiO2 scaffold struts. Progressive and sustained simvastatin release was observed for up to 19 days. No cytotoxic effects on osteoblasts were observed by scaffolds with simvastatin when compared to scaffolds without simvastatin. Expression of osteoblast markers (collagen type I alpha 1, alkaline phosphatase, bone morphogenetic protein 2, osteoprotegerin, vascular endothelial growth factor A and osteocalcin) was quantified using real-time reverse transcriptase–polymerase chain reaction. Secretion of osteoprotegerin, vascular endothelial growth factor A and osteocalcin was analysed by multiplex immunoassay (Luminex). The relative expression and secretion of osteocalcin was significantly increased by cells cultured on scaffolds with 10 μM simvastatin when compared to scaffolds without simvastatin after 21 days. In addition, secretion of vascular endothelial growth factor A was significantly enhanced from cells cultured on scaffolds with both 10 nM and 10 μM simvastatin when compared to scaffolds without simvastatin at day 21. In conclusion, the results indicate that simvastatin-coated TiO2 scaffolds can support a sustained release of simvastatin and induce osteoblast differentiation. The combination of the physical properties of TiO2 scaffolds with the osteogenic effect of simvastatin may represent a new strategy for bone regeneration in defects where immediate load is wanted or unavailable.

• Khanifam, Pejman; Pullisaar, Helen & Leknes, Knut N. (2013). Tannimplantater i ferske ekstraksjonsalveoler. Den norske tannlegeforenings tidende. ISSN 0029-2303. 123(3), s. 184–189.

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• Khanifam, Pejman; Pullisaar, Helen & Risheim, Helge (2020). Lokal blødtvævsatrofi i kinden og sensibilitetstab i højre side af overlæben efter subkutan ekstrusion af klorhexidindiglukonat i forbindelse med endodontisk behandling. Tandlægebladet. ISSN 0039-9353. Fulltekst i vitenarkiv
• Khanifam, Pejman; Pullisaar, Helen & Risheim, Helge (2019). Lokal bløtvevsatrofi i kinn og tap av følelse i høyre overleppe etter subkutan ekstrusjon av klorheksidindiglukonat i forbindelse med endodontisk behandling. Den norske tannlegeforenings tidende. ISSN 0029-2303. 129, s. 706–710.
• Pullisaar, Helen; Verket, Anders; Szoke, Krisztina; Tiainen, Hanna; Haugen, Håvard Jostein & Brinchmann, Jan E. [Vis alle 8 forfattere av denne artikkelen] (2014). Extracellular matrix coated ceramic scaffold enhance osteoblast differentiation.
• Pullisaar, Helen; Tiainen, Hanna; Landin, Maria Augusta Dos S Silva; Lyngstadaas, Ståle Petter; Haugen, Håvard Jostein & Reseland, Janne Elin [Vis alle 7 forfattere av denne artikkelen] (2013). Local delivery of simvastatin on titanium dioxide scaffolds; a tissue engineering model.
• Pullisaar, Helen; Tiainen, Hanna; Landin, Maria Augusta Dos S Silva; Lyngstadaas, Ståle Petter; Haugen, Håvard Jostein & Reseland, Janne Elin [Vis alle 7 forfattere av denne artikkelen] (2013). Local delivery of simvastatin enhances bone differentiation on porous titanium dioxide scaffold.
• Pullisaar, Helen; Tiainen, Hanna; Landin, Maria Augusta Dos S Silva; Lyngstadaas, Ståle Petter; Haugen, Håvard Jostein & Reseland, Janne Elin [Vis alle 7 forfattere av denne artikkelen] (2013). Enhanced in vitro bone differentiation on TiO₂ scaffold coated with simvastatin.
• Pullisaar, Helen; Tiainen, Hanna; Landin, Maria Augusta Dos S Silva; Lyngstadaas, Ståle Petter; Haugen, Håvard Jostein & Reseland, Janne Elin [Vis alle 7 forfattere av denne artikkelen] (2013). Coating titanium dioxide scaffold with simvastatin in alginate promote osteoblast differentiation in vitro.
• Khanifam, Pejman; Pullisaar, Helen & Leknes, Knut N. (2013). Implantater i friske ekstraktionsalveoler. Tandlægebladet. ISSN 0039-9353. 117(12), s. 1002–1008. Vis sammendrag

  Kliniske undersøgelser har dokumenteret høj succesrate ved traditionel implantatforankret protetik. Men trods mangeårig anvendelse er der stadig udfordringer knyttet til behandling med dentale implantater. Det er fortsat vanskeligt at opnå et tilfredsstillende æstetisk resultat ved erstatning af enkelttænder i overkæbens frontregion, og ingen kan angive det ideele tidspunkt for indsættelse af implantatet efter ekstraktion. Mange patienter oplever den lange behandlingstid med dårlige midlertidige løsniger som problematisk. Placering af implantater i friske ekstraktionealveoler er en behandlingsstrategi, som efter nøje vurdering og planlægning kan benyttes ved enkelttandsimplantater. Direkte indsættelse reducerer antallet af kirurgiske indgreb, giver kortere behandlingstid og er derfor et mere patientvenligt behandlingskoncept. Observationsstudier indikerer imidlertid, at direkte indsættelse oftere mislykkes, og det kan være vanskeligt at forudsige det postoperative blødt- og hårdtvævsniveau. Alveolens morfologi kan også vanskeliggøre primær stabilitet og optimal placering af implantatet. Denne artikel ønsker med en kort gennemgang af litteraturen at præsentere fordele og ulemper ved direkte indsættelse af implantater og diskutere, hvilke kliniske vurderinger der bør foretages, før denne behandlingsform kan anbefales. To dokumenterede patienttilfælde illustrerer forskellige kirurgiske teknikker og vigtige faser ved direkte indsættelse af tandimplantater i friske ekstraktionsalveoler.

• Pullisaar, Helen; Reseland, Janne Elin; Lyngstadaas, Ståle Petter & Haugen, Håvard Jostein (2012). Loading and release of simvastatin from titanium dioxide scaffolds.
• Mosti, Mats Peder; Stunes, Astrid Kamilla; Ericsson, Madelene; Reseland, Janne Elin; Pullisaar, Helen & Shabestari, Maziar [Vis alle 8 forfattere av denne artikkelen] (2012). Effects of the peroxisome proliferator-activated receptor (PPAR) delta agonist GW501516 on bone and muscle in ovariectomized rats.
• Pullisaar, Helen; Lyngstadaas, Ståle Petter; Haugen, Håvard Jostein & Reseland, Janne Elin (2010). Permanent bone graft materials for improved implant support and bone ingrowth in alveolar defects.

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