Peter Ninkov

• Ignjatović, Nenad L.; Ninkov, Peter; Sabetrasekh, Roya; Lyngstadaas, Ståle Petter & Uskoković, Dragan P. (2014). In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide. Bio-medical materials and engineering. ISSN 0959-2989. 24(4), s. 1647–1658. doi: 10.3233/BME-140978.
• Ninkov, Peter; Svanæs, Dagfinn Beyer & Bjørnland, Tore (2011). Anatomical changes of the mandibular canal, displaced root fragment and neurosensory deficit after removal of a mandibular third molar: report of a case. Oral Surgery. ISSN 1752-2471. 4, s. 140–144. doi: 10.1111/j.1752-248X.2011.2011.01123.x.
• Ingjatovic, Nenad L.; Ninkov, Peter; Sabetrasekh, Roya & Uskokovic, Dragan P. (2010). A novel nano drug delivery system based on tigecycline-loaded calciumphosphate coated with poly-dl-lactide-co-glycolide. Journal of materials science. Materials in medicine. ISSN 0957-4530. 21(1), s. 231–239. doi: 10.1007/s10856-009-3854-6. Vis sammendrag

  The purpose of the study presented in this paper has been to examine the possibility of the synthesis of a new nanoparticulate system for controlled and systemic drug delivery with double effect. In the first step, a drug is released from bioresorbable polymer; in the second stage, after resorption of the polymer, non-bioresorbable calcium phosphate remains the chief part of the particle and takes the role of a filler, filling a bone defect. The obtained tigecycline-loaded calcium-phosphate(CP)/poly(dl-lactide-co-glycolide)(PLGA) nanoparticles contain calcium phosphate coated with bioresorbable polymer. The composite was analyzed by FT-IR, XRD and AFM methods. The average particle size of the nanocomposite ranges between 65 and 95 nm. Release profiles of tigecycline were obtained by UV–VIS spectroscopy in physiological solution at 37°C. Experimental results were analyzed using Peppas and Weibull mathematical models. Based on kinetic parameters, tigecycline release was defined as non-Fickian transport. The cytotoxicity of the nanocomposite was examined on standard cell lines of MC3T3-E1, in vitro. The obtained low values of lactate dehydrogenase (LDH) activity (under 37%) indicate low cytotoxicity level. The behaviour of the composite under real-life conditions was analyzed through implantation of the nanocomposite into living organisms, in vivo. The system with the lowest tigecycline content proved to be an adequate system for local and controlled release. Having in mind the registered antibiotics concentration in other tissues, delivery systems with a higher tigecycline content show both local and systemic effects.

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